Hypolipidemic effects of a new piperine derivative GB-N from Piper longum in high-fat diet-fed rats

Figures & data

Figure 1.  Chemical structures of GB-N.

Table 1.  Sequences of primers used in RT-PCR.

Gene	Forward (5′–3″)	Reverse (5′–3″)
LDLR	ATCTCTCAGCTCCCAGTACTCCTA	AGAAGATGGACAGGAACCTCATAC
HMG-CoA	AAGGTGGTGAGAGAGGTGTTAAAG	AATACAGTTTGAACTCCCCACATT
LCAT	GAACTCAGTAACCACACACGG	TGCCAGACGCCAGTCATAGG
β-Actin	TGCTGTCCCTGTAGTCCTCT	AGGTCTTTACGGATGTCAACG

RT-PCR, real-time polymerase chain reaction; LDLR, low-density lipoprotein receptor; HMG-CoA, 3-hydroxy-3-methylglutaryl coenzyme A; LCAT, lecithin cholesterol acyltransferase.

Figure 2.  Effects of simvastatin, GB-N on the serum level of (A) total cholesterol (TC), (B) triglycerides (TG), (C) low-density lipoprotein cholesterol (LDL-C), and (D) high-density lipoprotein cholesterol (HDL-C). Compared with normal group, Δp < 0.05, ΔΔp < 0.01; compared with model group *p < 0.05, **p < 0.01.

Figure 3.  Effects of GB-N on levels of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA), lecithin cholesterol acyltransferase (LCAT) and low-density lipoprotein receptor (LDLR) mRNA determined by real-time polymerase chain reaction (RT-PCR). Compared with model group, *p < 0.05, **p < 0.01.

Figure 4.  Effects of simvastatin, GB-N (2.5, 5, 10 mg/kg) on level of lecithin cholesterol acyltransferase (LCAT), low-density lipoprotein receptor (LDLR) and CYP7A1 protein expression determined by western blot. Compared with model group, *p < 0.05, **p < 0.01.