The evaluation of topical administration of Bellis perennis fraction on circular excision wound healing in Wistar albino rats

Figures & data

Figure 1.  Histological sections of cutaneous wound site obtained from the controls, HOT and HOTBp lesions of the rats (A) normal histological skin tissue in the first day. (H&E, ×200) (B) normal histological skin tissue in the first day samples stained with Gomori trichrome stain (×200); (C) Late phase granulation tissues which were characterized with fibroblastic proliferation and scattered lymphocytes in a fifth day subject. Normal dermal tissue is seen at right corner [(rectangular) (Hematoxylin and eosin, ×100); (D) The collagen fibers started to emerge slightly in the granulation tissue in the 5^th day samples (Gomori trichrome stain, ×100); (E) a lesion at a 10^th day subject. Collagen fibrils is seen as more abundant than the 10^th day sample (Gomori trichrome stain, ×100), (F) a sample of control lesion in the 30^th day. Healing with fibrous scar formations is seen (Hematoxylin and eosin, ×100) (G) same tissue of F with Gomori trichrome stain (×100) (H, J) Near normal skin tissue without fibros scar tissue seen in a 30^th day sample treated with Bellis perennis (Hematoxylin and eosin, Gomori trichrome stain, respectively, ×100). (e) epidermis, (d) dermis, (ndt) normal dermal tissue, (cf) collagen fibers.

Figure 2.  Wound closure percentages of the control, HOT and HOTBp groups at the 5^th, 10^th and the 30^th days of the experiment.

Figure 3.  Photographs of the different rats in different days of the experiment. HOT: hydrophilic ointment treatment, HOTBp: hydrophilic ointment loaded with B. perennis fraction.