Antitumor activity and antioxidant property of Curcuma caesia against Ehrlich’s ascites carcinoma bearing mice

Figures & data

Figure 1. Cytotoxic effect of MECC on in vitro EAC cell line. Values are mean ± S.E.M.; where n = 3.

Table 1. Effect of MECC on tumor volume, tumor weight, total cell count, viable and nonviable cell count, MST and %ILS in EAC bearing mice.

Parameters	EAC control	EAC + MECC (50 mg/kg)	EAC + MECC (100 mg/kg)	EAC + 5-FU (20 mg/kg)
Tumor volume (mL)	2.75 ± 0.13	1.40 ± 0.13*	0.91 ± 0.05*	0.53 ± 0.05*
Tumor weight (g)	3.21 ± 0.10	1.08 ± 0.08*	0.83 ± 0.06*	0.57 ± 0.04*
Total cell (×10^7 cell/mL)	9.30 ± 0.43	4.00 ± 0.13*	3.89 ± 0.05*	3.81 ± 0.09*
Viable cell (×10^7 cell/mL)	8.92 ± 0.42	2.78 ± 0.18*	0.94 ± 0.08*	0.60 ± 0.08*
Nonviable cell (×10^7 cell/mL)	0.38 ± 0.04	1.22 ± 0.07*	2.96 ± 0.08*	3.21 ± 0.09*
Viable %	95.91	69.50	24.16	15.75
Nonviable %	4.09	30.50	76.09	84.25
MST (days)	21	33	39.5	43
% ILS	00	57.14	88.09	104.76

Values are represented as mean ± SEM, where n = 6. *p < 0.01 for treated groups versus EAC control group.

Table 2. Effect of MECC on hematological parameters in EAC bearing mice.

Parameters	Normal control	EAC control	EAC + MECC (50 mg/kg)	EAC + MECC (100 mg/kg)	EAC + 5-FU (20 mg/kg)
RBC (cell × 10^6/μL)	5.89 ± 0.20	2.93 ± 0.11^a,*	4.81 ± 0.23^b,*	5.09 ± 0.70^b,*	5.19 ± 0.20^b,*
WBC (cell × 10^3/μL)	5.16 ± 0.32	10.97 ± 0.68^a,*	7.53 ± 0.36^b,*	6.16 ± 0.27^b,*	5.09 ± 0.33^b,*
Hb (g/dL)	11.48 ± 0.98	4.80 ± 0.29^a,*	7.98 ± 0.41^b,*	9.86 ± 0.41^b,*	10.35 ± 1.16^b,*

Values are represented as mean ± SEM, where n = 6.

^aEAC control group versus normal group.

^bTreated groups versus EAC control group, *p < 0.01.

Figure 2. Effect of MECC on serum biochemical parameters such as total protein count (a), SGOT (b), SGPT (c) and ALP (d) in EAC bearing mice. Values are represented as mean ± SEM, where n = 6. ^aEAC control group versus normal control group, ^#p < 0.01. ^bAll treated groups versus EAC control group, *p < 0.01.

Figure 3. Effect of MECC on tissue antioxidant defense parameters like total protein count (a), protein oxidation (b), lipid peroxidation (c), CAT (d), reduce GSH (e) and SOD (f) in EAC bearing mice. Values are represented as mean ± SEM, where n = 6. ^aEAC control group versus normal control group, ^#p < 0.01. ^bAll treated groups versus EAC control group, *p < 0.01.