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Research Article

Study on hemostatic activities of the rhizome of Paris bashanensis

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Pages 1321-1325 | Received 02 Sep 2012, Accepted 22 Mar 2013, Published online: 16 Jul 2013

Figures & data

Figure 1. Regulatory effects of 30, 50 and 70% n-BuOH ext. on bleeding and coagulation time. Mice were treated orally with the water solvent (control group), Yunnan baiyao powder (150 mg/kg, positive group), and 30, 50 and 70% n-BuOH ext. (100 mg/kg, 50 mg/kg) for 5 days. (A) The measurement of blood clotting was represented by the bleeding time from the cut tail. (B) The blood coagulation time was determined by using the slide methods. (C) The blood coagulation time was determined by using the capillary methods. Data were expressed as time of bleeding or blood coagulation (s), mean ± SEM, n = 10, *p < 0.05, ** p < 0.01 compared with the control group.

Figure 1. Regulatory effects of 30, 50 and 70% n-BuOH ext. on bleeding and coagulation time. Mice were treated orally with the water solvent (control group), Yunnan baiyao powder (150 mg/kg, positive group), and 30, 50 and 70% n-BuOH ext. (100 mg/kg, 50 mg/kg) for 5 days. (A) The measurement of blood clotting was represented by the bleeding time from the cut tail. (B) The blood coagulation time was determined by using the slide methods. (C) The blood coagulation time was determined by using the capillary methods. Data were expressed as time of bleeding or blood coagulation (s), mean ± SEM, n = 10, *p < 0.05, ** p < 0.01 compared with the control group.

Figure 2. HPLC chromatograms of 30 (A), 50 (B), 70% (C) n-BuOH ext. of P. bashanensis. The peaks corresponding to Paris VII (1), PGRR {(25R)-5-en-spirost-3,17-diol-3-O-α-l-rhamnopyranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyanoside} (2), Paris H (3), Paris VI (4), Paris II (5), Gracillin (6) and Paris I (7) were identified.

Figure 2. HPLC chromatograms of 30 (A), 50 (B), 70% (C) n-BuOH ext. of P. bashanensis. The peaks corresponding to Paris VII (1), PGRR {(25R)-5-en-spirost-3,17-diol-3-O-α-l-rhamnopyranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyanoside} (2), Paris H (3), Paris VI (4), Paris II (5), Gracillin (6) and Paris I (7) were identified.

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