Figures & data
Table 1. Effect of solvent fractions isolated from Kalanchoe pinnata on blood glucose levels.
Table 2. Effect of Kalanchoe pinnata DCM fraction on biochemical parameters.
Figure 1. Effect of solvent fractions on insulin secretion from isolated rat islets. Insulin secretion induced by 11.8 mM glucose was considered as a negative control and glibenclamide (10 µg/ml) as a positive control. All the extracts were used at a concentration 10 µg/ml. Results are mean ± S.D.; n = 6. *p < 0.05 and **p < 0.001 significant from 11.8 mM glucose control.
Figure 2. Effect of basal glucose (2.8 mM) on insulin secretagogue activity of the DCM fraction. Results are mean ± S.D.; n = 6. *p < 0.05 and **p < 0.001significant from 2.8 mM glucose control.
Figure 3. Probable mechanism for insulin secretagogue action of DCM fraction. Effect of 300 µM diazoxide (3A) and 20 µM nifedipine (3B) on insulin secretagogue activity of the Kalanchoe pinnata DCM fraction (10 µg/ml). Results are mean ± S.D.; n = 6. *p < 0.05 and **p < 0.001significant from 2.8 mM glucose control.
Figure 4. HPTLC separation of the bioactive DCM fraction. The bioactive DCM fraction was separated by using methanol:glacial acetic acid (95:5 v/v). Only two peaks were observed at Rf 0.74 and 0.89.
Figure 5. GC-MS analysis of the bioactive component from DCM fraction. GC-MS of the DCM fraction (5A) and mass fragmentation pattern (5B) of the major component at RT 15.478.
Figure 6. FTIR spectra of the bioactive component from DCM fraction.
