Accelerated sample preparation and formation of astragaloside IV in Astragali Radix

Figures & data

Figure 1. Structures of astragaloside I, II, IV, malonylastragaloside I from A. mongohlicus and methyl-malonylastragaloside I.

Table 1. Comparison of ¹H and ¹³C NMR data (600 and 150 MHz, respectively) of methyl-malonyl-AGS-I (pyridine d₅, 23 °C, 5) with published data of malonyl-AGS-I (3).

	Comp. 5		Comp. 3 (Chu et al., 2010)
Pos.	δC	δH	δC	δH
1	31.9	1.51/1.23	32.0
2	29.7	2.15/1.87	29.6
3	89.2	3.37	89.4	3.35
4	42.5	–	42.2
5	52.3	1.86	52.4	1.83
6	79.2	3.78	79.3	3.75
7	34.8	2.33/1.80	34.9
8	46.0	1.93	46.0
9	21.1	–	21.2
10	28.8	–	28.9
11	26.1	1.83/1.23	26.1
12	33.4	1.62/1.57	33.4
13	45.0	–	45.0
14	46.1	–	46.2
15	46.3	2.36/1.85	46.3
16	73.3	5.00	73.4	4.97
17	58.2	2.53	58.3	2.51
18	21.1	1.42	21.1	1.41
19	29.1	0.22/0.57	29.2	0.20/0.55
20	87.2	–	87.2
21	28.4	1.31	27.1	1.29
22	34.8	3.14/1.68	34.8	1.69
23	26.4	2.33/2.05	26.4	3.14*
24	81.7	3.89	81.7	3.88
25	71.2	–	71.3
26	28.2	1.58	28.2	1.57
27	27.0	1.31	28.3	1.29
28	28.3	1.78	28.5	1.76
29	16.3	1.25	16.4	1.23
30	19.8	0.93	19.9	0.92
1′	105.1	4.86	103.6	4.84
2′	72.2	5.47	72.4	5.42
3′	72.2	5.71	72.4	5.74
4′	70.5	5.41	70.3	5.47
5′	62.1	4.30/3.63	62.5	4.34/3.65
1’’	105.1	4.93	105.2	4.90
2′′	75.5	4.05	75.6	4.02
3′′	79.1	4.22	79.2	4.19
4′′	71.8	4.21	71.9	4.18
5′′	78.1	3.93	78.1	3.89
6′′	63.0	4.50/4.33	63.1	4.46/4.30
2′-Acetyl	169.5	–	169.6
	20.6	2.02	20.7	2.00
3′-Acetyl	170.1	–	170.2
	20.5	2.08	20.6	2.06
4′-MeMal.	166.2	–	167.1
	41.4	3.68	42.6	3.73
	167.0	–	169.3
	52.3	3.63

*Wrong assignment.

Figure 2. HPLC–ELSD chromatogram of the methanol extract of Astragali Radix according to the method of the (A) European Pharmacopoeia (Pharm. Eur.) and (B) the accelerated method.

Figure 3. HPLC-ELSD chromatograms of the methanol extract (accelerated extraction) of Astragali Radix before (A) and after (B) treatment with ammonia R (four washing steps).

Figure 4. HPLC–ELSD chromatograms of a reference mixture of astragaloside I (AGS-I) (1), AGS-II (2) and methyl-malonyl-AGS-I (5) before (A) and after (B) treatment with ammonia R.

Chu C, Cai H-X, Ren M-T, et al. (2010). Characterization of novel astragaloside malonates from Astragali Radix by HPLC with ESI quadropole TOF MS. J Sep Sci 33:570–81

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