Treated effect of silymarin on vascular function of aged rats: Dependant on nitric oxide pathway

Figures & data

Figure 1. Concentration-response curves for phenylephrine in intact endothelium aortic rings of control rats (young) and of old rats. Some old animal rings were incubated with SM (50 mg/L), SM + l-NAME (100 µM) or SM + INDO (10 µM). Values are mean ± SEM of eight or nine rings. ^#p < 0.001 versus young group.

Table 1. Contractile responses to phenylephrine and KCl (milligram/dry tissue weight) and relaxant responses to acetylcholine and SNP (% phenylephrine precontraction) in intact endothelium aortic rings of control rats (young) and old rats.

	Phenylephrine		KCl	Acetylcholine		Sodium nitroprussid
	Emax	pD2	Emax	Emax	pD2	Emax	pD2
Young	712 ± 89	6.45 ± 0.08	882 ± 118.4	93.9 ± 2.09	7.64 ± 0.07	99.6 ± 1.52	7.47 ± 0.07
Old	718 ± 75	6.88 ± 0.09**	499 ± 80.4*	72.0 ± 2.56***	7.09 ± 0.19***	100 ± 0.62	7.23 ± 0.06*
Old + SM	548 ± 109	6.65 ± 0.06	223 ± 48.9***	86.3 ± 1.90*	7.00 ± 0.16***	101 ± 2.06	7.88 ± 0.09***
Old + SM + l-NAME	1266 ± 114***	7.02 ± 0.07***	615 ± 76.2	26.1 ± 6.16***	7.19 ± 0.22***	103 ± 2.62	7.95 ± 0.10***
Old + SM + INDO	410 ± 64*	6.60 ± 0.06	161 ± 39.5***	88.4 ± 2.79	6.90 ± 0.19**	101 ± 2.32	7.78 ± 0.18

Some old animal rings were incubated with SM (50 mg/L), SM + l-NAME (100 µM) or SM + INDO (10 µM). E_max denotes maximum contraction and pD₂ represents –logEC₅₀. Values are mean ± SEM of eight or nine rings. *p < 0.05, **p < 0.01, ***p < 0.001 versus young group.

Figure 2. Concentration-response curves for acetylcholine in intact endothelium aortic rings of control rats (young) and old rats. Some old animal rings were incubated with SM (50 mg/L), SM + l-NAME (100 µM) or SM + INDO (10 µM). Values are mean ± SEM of eight or nine rings. ^#p < 0.001 versus young group.

Figure 3. Concentration-response curves for SNP in intact endothelium aortic rings of control rats (young) and old rats. Some old animal rings were incubated with SM (50 mg/L), SM + l-NAME (100 µM) or SM + INDO (10 µM). Values are mean ± SEM of eight or nine rings. *p < 0.05 versus young group.