Figures & data
Figure 1. Morphologic changes of PC-3 cells treated with various concentrations of formononetin for 48 h. (A) Representative image of cells from the non-treated group. (B–D) Representative images of cells treated with 25, 50 and 100 μM formononetin, respectively. The apoptotic cells showed condensed nuclei or apoptotic bodies (indicated by arrows). The images were taken using an Olympus IX71FL fluorescence microscope (400×).
Figure 2. Effect of formononetin treatment on Bax mRNA expression levels in PC3 cells. Bax mRNA expression was significantly increased with 50 and 100 μM formononetin treatments. The results of real-time PCR are presented as mean ± SD for triplicate experiments. *p < 0.05 versus non-treated group.
Figure 3. Western-blot analysis of Bax, IGF-1 R and p-IGF-1 R protein levels with formononetin treatment. (A) Formononetin increased the expression levels of Bax protein in a dose-dependent manner. β-Actin was used as the loading control. (B) Formononetin inhibited the expression of p-IGF-1 R protein in a dose-dependent manner. IGF-1 R protein was used as the loading control. The quantification of the western-blot assays are presented as mean ± SD for triplicate experiments.*p < 0.05 versus non-treated group.
