Figures & data
Figure 1. Contractile response to increasing calcium (Ca2+) concentrations (0.39–1.81 mM) in ileum preparations. The tissues were depolarized with KCl (50 mM) and initially maintained in Ca2+free medium in the absence or in the presence of crude extracts (n = 4). Data are expressed as the % of the maximal contractile response to CaCl2. (*) Indicates a statistically significant difference based on the ANOVA of repeated measures and the post-hoc Duncan test (p ≤ 0.05). Values shown are the mean ± SEM (standard error of the mean) of six determinations. In all the experiments, the inhibitory effects of Aq, Hx and Me were reversed after 0.5 h of successive watch out.
Figure 2. Antispasmodic effect of the crude extracts and the methanolic fractions of A. repens leaves on the CaCl2 contractions of rat ileum slices. (*) Indicates a statistically significant difference with the one-way ANOVA and the post-hoc Duncan test (p ≤ 0.05). Values shown are the mean ± SEM (standard error of the mean) of four determinations.
Figure 3. Contractile responses to potassium (K+) increasing concentrations (0.98–4.54 mM) in ileum preparations. The tissues were prepared with K+-free Tyrodes solution in the absence (n = 4) or in the presence of crude extracts. Data are expressed as the % of the maximal contractile response to KCl. (*) Indicates statistically significant difference with the ANOVA of repeated measures and the post-hoc Duncan test (p ≤ 0.05). Values shown are the mean ± SEM. In all the experiments, the inhibitory effects of Aq, Hx and Me were reversed after 0.5 h of successive watch out.
Figure 4. Spasmolytic effect of the crude extracts and the methanolic fractions of A. repens on the KCl contractions of rat ileum slices. (*) Indicates a statistically significant difference with one-way ANOVA and the post-hoc Duncan test (p ≤ 0.05). Values shown are the mean ± SEM (standard error of the mean) of four determinations.
Figure 5. Contractile responses to increasing 5-HT concentrations (2.23 × 10−6–10.25 × 10−6 M) in ileum preparations. The tissues were prepared with Tyrodes solution in the absence or in the presence of crude extracts (n = 4). Data are expressed as the % of the maximal contractile response to 5-HT. (*) Indicates a statistically significant difference with the ANOVA of repeated measures and the post-hoc Duncan test (p ≤ 0.05). Values shown are the mean ± SEM. In all the experiments, the inhibitory effect of Aq, Hx and Me were was reversed after 0.5 h of successive watch out.
Figure 6. Spasmolytic effect of the crude extracts and the methanolic fractions of A. repens leaves on the 5-HT contractions of rat ileum slices. (*) Indicates a statistically significant difference (p ≤ 0.05). Values shown are the mean ± S.E.M. (standard error of the mean) of four determinations.
