Efficient protocol for rapid Aloe vera micropropagation

Figures & data

Figure 1. The effect of different concentrations and combinations of plant growth regulators BAP, Kin, IAA, and NAA on the number of shoots per explant (A), shoot length (B), root length (C), shoot leaf diameter, and (D) in vitro propagation of A. vera.

Table 1. Effects of different concentrations and combinations of BAP, IAA, Kin, and NAA on multiple shoot formation in A. vera.

Growth regulators (mg/l)	NSE	SL (cm)	RL (cm)	SLD (cm)
IAA(0.2) + BAP(0.8)	48.03 ± 5.83^a	5.98 ± 2.2^a	7.45 ± 2.2^b	0.55 ± 0.01^b
IAA(0.1) + Kin(1)	21.86 ± 1.67^b	6.37 ± 0.9^a	9.51 ± 2.3^a	0.60 ± 0.01^a
IAA(1) + BAP(4)	58.88 ± 6.05^a	5.27 ± 1.8^b	5.89 ± 2.5^c	0.51 ± 0.01^b
NAA(0.5) + BAP(2)	22.16 ± 3.24^b	5.26 ± 1.3^b	5.63 ± 1.6^c	0.55 ± 0.01^b

Different letters in each column indicate significant differences at a 1% level

where NSE (shoots/explant), SL (shoot length), RL (root length), and SLD (shoot leaf diameter).

Figure 2. In vitro plantlet A. vera having healthy shoots and roots.

Figure 3. In vitro regenerated A. vera.

Figure 4. Adaptation of micropropagation plantlets A. vera and transferring them into soil.

Figure 5. Acclimatized A. vera in pot conditions.