Inhibitory effects of phenolic compounds from Artocarpus styracifolius on respiratory burst of rat neutrophils

Figures & data

Figure 1. Chemical structures of AS and QC (positive control).

Figure 2. Inhibitory effects of AS on rat PMNs respiratory burst activated by PMA. (A) Dynamics curve of rat neutrophils-CL reaction stimulated by PMA. (B) Inhibitory effects of 20 µM of AS and QC (positive control) on PMA-stimulated respiratory burst of rat PMNs. Data are means ± SD of four independent experiments. *p < 0.05 (Turkey’s test) in comparison with that of control. NS indicates non-significant. (C) Inhibitory effects of AS1–6 on PMA-induced respiratory burst of rat PMNs at various concentrations (5–20 µM). Data are means ± SD of four independent experiments.

Table 1. The selectivity of anti-respiratory burst properties of phenolics from A. styracifolius.

Compounds	Inhibiting PMNs respiratory burst (IC50, µM)	Cytotoxicity (CC50, µM)	SI^a
QC	4.7 ± 0.3	226.2 ± 19.2	48.1
AS1	3.1 ± 0.3	198.7 ± 11.6	64.1
AS2	9.1 ± 0.8	1784.1 ± 101.2	196.1
AS3	10.0 ± 1.1	209.2 ± 14.5	20.9
AS4	57.7 ± 6.6	826.5 ± 28.2	14.3
AS5	29.7 ± 2.3	2588.2 ± 124.3	87.1
AS6	5.9 ± 0.6	1501.3 ± 104.8	254.5

^aSI = CC₅₀/IC₅₀.

Figure 3. The scavenging capacity of 20 µM of AS1–6 and QC (positive control) towards , H₂O₂, and HO^• in acellular systems. Data are means ± SD of four independent experiments. *p < 0.05 (Turkey’s test) in comparison with that of control. NS indicates non-significant.