Curcumin attenuates fructose-induced vascular dysfunction of isolated rat thoracic aorta rings

Figures & data

Figure 1. Effect of curcumin alone (1 µM) or in combination with tempol (1 mM) or 1400 W (1 µM) on fructose-treated isolated rat aorta responsiveness to KCl. Symbols indicate mean ± SEM for n = 6–10 aortic rings; ***p < 0.001, compared with the corresponding control group values; ###p < 0.001 compared with the corresponding fructose group values; ^aap < 0.001 compared with curcumin group values.

Figure 2. Effect of curcumin alone (1 µM) or in combination with tempol (1 mM) or 1400 W (1 µM) on fructose-treated isolated rat aorta responsiveness to phenylephrine (PE). Symbols indicate mean ± SEM for n = 6–10 aortic rings; ***p < 0.001, compared with the corresponding control group values; ##p < 0.001 compared with the corresponding fructose group values; ^ap < 0.01 compared with curcumin group values.

Table 1. Effect of fructose and curcumin either alone or in combination with either tempol or 1400 W on the maximal response (Emax) and pD2 (−Log EC₅₀) values of phenylephrine (PE), potassium chloride (KCl), and acetyl choline (Ach), dose–response curves.

Treatment	Control	Fructose (F)	F + curcumin	F + curcumin + tempol	F + curcumin + 1400 W
KCl
Emax	344.0 ± 12.9	517.6* ± 21.55	399.8** ± 16.66	411.9** ± 19.36	314.9** ± 11.72
pD2	40.00 ± 0.19	39.87 ± 0.23	30.00** ± 0.22	29.74** ± 0.27	20.83** ± 0.48
PE
Emax	477.0 ± 24.12	702.6* ± 38.42	569.2** ± 31.23	518.3** ± 38	445.5** ± 25.18
pD2	6.48 ± 0.13	6.59 ± 0.16	6.59 ± 0.14	7.07 ± 0.22	6.60 ± 0.15
Ach
Emax	59.27 ± 4.71	44.92 ± 3.32	34.62 ± 3.35	22.80 ± 7.04	37.97 ± 3.13
pD2	6.095 ± 0.13	8.18 ± 1.06	6.13 ± 0.17	6.095 ± 0.55	6.44 ± 0.16

Values are expressed as mean ± standard error of mean, n = 6–10 aortic rings.

*p < 0.05, compared with the corresponding control group values; **p < 0.05 compared with the corresponding fructose group values; by a one-way analysis of variance and Bonferroni post hoc test.

Figure 3. Effect of curcumin alone (1 µM) or in combination with tempol (1 mM) or 1400 W (1 µM) on fructose-treated isolated rat aorta responsiveness to acetylcholine (Ach). Symbols indicate mean ± SEM for n = 6–10 aortic rings.

Figure 4. Effect of curcumin (1 µM) on intracellular calcium in fructose-treated isolated rat aorta. Symbols indicate mean ± SEM for n = 6–10 aortic rings; *p < 0.05, compared with the corresponding control group values; **p < 0.05 compared with the corresponding fructose group values.

Figure 5. Effect of curcumin (1 µM) on area under the calcium curve (AUC) intracellular calcium in fructose-treated isolated rat aorta. Symbols indicate mean ± SEM for n = 6–10 aortic rings; *p < 0.05, compared with the corresponding control group values; **p < 0.05 compared with the corresponding fructose group values.

Figure 6. Effect of curcumin (1 µM) on intracellular nitric oxide (NO) in fructose-treated isolated rat aorta. Symbols indicate mean ± SEM for n = 6–10 aortic rings.