Gastroprotective effect of standardized leaf extract from Careya arborea on experimental gastric ulcers in rats

Figures & data

Figure 1. (a) HPTLC profile of CALE under UV 366 nm. (b) HPTLC chromatogram of CALE at a scan wavelength of 278 nm. T₁: standard ellagic acid; T₂: standard gallic acid; T₃: standard quercetin.

Figure 2. Effect of CALE on H⁺K⁺ATPase activity in gastric mucosa and gastric wall mucus in EtOH-induced ulcer group. +p < 0.001 compared to the respective normal control group. ^ap < 0.05, ^bp < 0.01 and ^cp < 0.001 compared to the respective EtOH-induced ulcer group.

Table 1. Gastroprotective activity of CALE on aspirin-, EtOH-, CRS-, and PL-induced ulcers.

	ASP-induced ulcers		EtOH-induced ulcers		CRS-induced ulcers		PL-induced ulcers
Treatment	Ulcer index	Protective	Ulcer index	Protective	Ulcer index	Protective	Ulcer index	Protective
(mg/kg)	(mm^2/rat)	ratio (%)	(mm^2/rat)	ratio (%)	(mm^2/rat)	ratio (%)	(mm^2/rat)	ratio (%)
Control	12.4 ± 0.8	–	14.2 ± 0.62	–	15.82 ± 2.12	–	10.20 ± 0.10	–
CALE 100	10.8 ± 1.2	12.90	12.5 ± 0.80	11.97	9.76 ± 1.89^a	38.30	7.28 ± 0.61^a	28.63
CALE 200	8.2 ± 0.8^a	33.87	10.90 ± 0.93^a	23.24	7.83 ± 1.12^b	50.50	5.92 ± 0.41^a	41.96
CALE 400	6.0 ± 1.5^b	51.61	8.47 ± 1.52^b	40.35	5.32 ± 1.43^c	66.37	3.68 ± 1.92^c	63.92
RAN 50	5.3 ± 1.1^b	57.26	4.62 ± 0.31^c	67.46	3.86 ± 0.73^c	75.60	2.71 ± 0.83^c	73.43

Mean ± S.E.M. for six rats.

^ap < 0.05, ^bp < 0.01, ^cp < 0.001 compared to the respective control group.

Table 2. Effect of CALE on gastric secretion in 4h PL rats: effect on volume, acid, and pepsin.

		Acid		Peptic
Treatment	Volume	Concentration	Output	Concentration	Output
(mg/kg)	(ml/100 g)	(µEq/ml)	(µEq/4 h)	(µmol/ml)	(µmol/h)
Control	185 ± 0.26	84.2 ± 12.4	246.4 ± 34.8	286.8 ± 28.3	658.2 ± 48.2
CALE 400	152.0 ± 0.46^c	68.7 ± 13.7	128.5 ± 29.4^a	258.9 ± 27.1	329.7 ± 58.3^b
RAN 50	149.0 ± 1.70^c	65.4 ± 12.1	135.4 ± 23.8^a	189.4 ± 21.7^a	428.5 ± 61.3^a

Mean ± S.E.M. for six rats.

^ap < 0.05, ^bp < 0.01, ^cp < 0.001 compared to the respective control group.

Figure 3. Effect of CALE on LPO, SOD and CAT activity in rat gastric mucosa in CRS-induced gastric ulcers in rats. +p < 0.001 compared to the respective control group. ^ap < 0.05, ^bp < 0.01 and ^cp < 0.001 compared to the respective CRS group.