Simultaneous quantification by HPLC of the phenolic compounds for the crude drug of Prunus serotina subsp. capuli

Figures & data

Figure 1. Typical HPLC chromatogram of the infusion obtained from the leaves of P. serotina. (a) Hyperoside (1); (b) chlorogenic acid (2); (c) and (d) Ps-1 and Ps-4 samples, respectively.

Table 1. Validation parameters evaluated for quantifying 1–3 in P. serotina using the developed HPLC method.

	LC method
Parameter	1	2	3
r^2 (n = 6)^a	0.9986	0.9968	0.9985
Regression equation	25446x + 174439	11128x + 30447	64095x + 862067
Working range, µg mL^−1	100–1000	100–1000	100–500
LOD, µg mL^−1 (n = 3)^b	8.8	4.5	10.5
LOQ, µg mL^−1 (n = 3)^c	15.4	14.5	13.1
Average recovery, % (n = 3)	99.8–100.9	99.5–101.0	98.9–101.2
Recovery RSD, % (n = 3)^d	0.9	0.6	1.3
Reproducibility RSD, % (n = 3)	0.4	0.6	1.2
Theoretical plates	2197	2363	2693
Symmetry factor	1.3	1.3	1.8
Tailing factor	1.12	1.11	1.23
Resolution	2.9	3.9	2.6

^ar² = determination coefficient.

^bLOD = limit of determination, 3.3 × (SD of the response/slope of the calibration curve).

^cLOQ = limit of quantification, 10 × (SD of the response/slope of the calibration curve).

^dRSD = relative standard deviation.

Table 2. Content of hyperoside (1), chlorogenic acid (2), and benzaldehyde (3) in P. serotina.

		Content (µg g^−1)^b
Sample^a	Source (year of harvest)	1	2	3
Ps-1	Market I (2011)	166.9 ± 5.25	500.5 ± 10.33	114.1 ± 6.94
Ps-2	Market II (2011)	162.1 ± 8.45	433.8 ± 13.64	106.4 ± 7.82
Ps-3	Market III (2011)	164.0 ± 2.58	516.5 ± 10.57	127.7 ± 5.55
Ps-4	Market IV (2010)	288.8 ± 12.6	503.0 ± 14.47	119.4 ± 9.92
Ps-5	Market V (2010)	201.6 ± 6.52	480.8 ± 15.67	105.6 ± 8.54
Ps-6	Market VI (2010)	130.8 ± 2.01	444.9 ± 19.03	136.1 ± 6.32

^aPs-1 to Ps-6 leaves of P. serotina.

^bThe value is the mean ± SD (n = 3)