Figures & data
Figure 1. Structural formula of EJ-01.
Figure 2. Mice were treated with the indicated dose of EJ-01(PO) and etoricoxib (PO) 30 min before chemical nociceptive stimuli (formalin 2.5% v/v; 20 µL; intraplantar). Observations (number of flinches/lickings) were recorded at (0–5 min) early phase (A) and (15–30 min) late phase (B). Acetic acid writhing test (acetic acid 3% v/v; 300 mg/kg IP) observations (number of writhes) were recorded over a period of 20 min after stimuli (C). Bars (mean ± SE; six animals were kept in each group) with dissimilar superscript differ significantly (p < 0.05).
Figure 3. Mice were treated with the indicated dose of EJ-01(PO) and morphine (IP) 30 min before thermal nociceptive stimuli (hot plate test). Observations (jumping/hind paw flinching/hind paw licking) were recorded at 1 h (A), 3 h (B), and 5 h (C) of test. Tail flick test observations (tail withdrawal latency) were recorded at 1 h (D), 3 h (E), and 5 h (F) of test. Bars (mean ± SE; six animals were kept in each group) with dissimilar superscript differ significantly (p < 0.05).
Table 1. Effect of EJ-01 on RAW 264.7 cells viability (MTT assay).
Figure 4. RAW 264.7 cells were co-incubated with the indicated concentrations of EJ-01 and LPS (2 µg mL−1) for 24 h in 10% phenol red-free DMEM medium containing 2% FCS. Naïve control cells were neither treated with EJ-01 nor stimulated with LPS. Vehicle control cells were incubated with vehicle alone. The culture supernatants were analyzed for NOx (A), TNF-α (B), and IL-1β (C) by ELISA. Bars (mean ± SE from three separate experiments) with dissimilar superscript differ significantly (p < 0.05).
