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Research Article

Mucor fragilis as a novel source of the key pharmaceutical agents podophyllotoxin and kaempferol

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Pages 1237-1243 | Received 01 Aug 2013, Accepted 15 Jan 2014, Published online: 27 May 2014

Figures & data

Table 1. Endophytic fungal strains and their TLC screening results.

Figure 1. Morphological characteristics of strain TW5: colony on a PDA plate (a); microscopic examination of the structures of mature sporangium and sporangiophore (200×) (b); and the shape of the sporangiospore (400×) (c).

Figure 1. Morphological characteristics of strain TW5: colony on a PDA plate (a); microscopic examination of the structures of mature sporangium and sporangiophore (200×) (b); and the shape of the sporangiospore (400×) (c).

Figure 2. Neighbor-joining trees based on the partial ribosomal gene sequences of strain TW5 and its closest relatives. The scale bar indicates a 2% estimated difference in the nucleotide sequence. GenBank accession numbers are shown in parentheses.

Figure 2. Neighbor-joining trees based on the partial ribosomal gene sequences of strain TW5 and its closest relatives. The scale bar indicates a 2% estimated difference in the nucleotide sequence. GenBank accession numbers are shown in parentheses.

Figure 3. Growth curve of M. fragilis (strain TW5). The error bars represent the standard deviations from the mean value of three flasks.

Figure 3. Growth curve of M. fragilis (strain TW5). The error bars represent the standard deviations from the mean value of three flasks.

Figure 4. HPLC chromatograms of standard podophyllotoxin (a), fungal podophyllotoxin (b), standard kaempferol (c), and fungal kaempferol (d).

Figure 4. HPLC chromatograms of standard podophyllotoxin (a), fungal podophyllotoxin (b), standard kaempferol (c), and fungal kaempferol (d).

Figure 5. Chemical structures of podophyllotoxin (a) and kaempferol (b). Numbers represent the carbon positions.

Figure 5. Chemical structures of podophyllotoxin (a) and kaempferol (b). Numbers represent the carbon positions.

Table 2. 1H- and 13C-NMR characterization of fungal podophyllotoxin.

Table 3. 1H- and 13C-NMR characterization of fungal kaempferol.

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