DaoTan decoction (DTD) inhibits tumor necrosis factor-α (TNF-α)-induced expression of intercellular adhesion molecule-1 (ICAM-1), p53 and p21, in human umbilical vein endothelia cells (HUVECs)

Figures & data

Table 1. Sequences of primers used for RT-PCR.

Name	Oligo	Primer sequence	Predicted size (bp)	Genebank accession
ICAM-1	Forward primer	5′-AAACGGGAGATGAATGGT-3′	190	NM_012967
	Reverse primer	5′-TCTTCCTCTGGCGGTAAT-3′
p53	Forward primer	5′-GCTGAGTATCTGGACGACA-3′	224	NM_030989
	Reverse primer	5′-CAGGCACAAACACGAACC-3′
p21	Forward primer	5′-CAGAGCCACAGGCACCAT-3′	174	U24174
	Reverse primer	5′-GCGAAGTCAAAGTTCCACC-3′
β-Actin	Forward primer	5′-ACGTTGACATCCGTAAAGAC-3′	203	NM_031144
	Reverse primer	5′-CTGGAAGGTGGACAGTGAG-3′

Figure 1. The mRNA expression of ICAM-1, p53 and p21. The cells were pretreated with DTD serum (5, 10, and 20%, 6 h), PFT-α (25 μM, 30 min), and then co-treated with TNF-α (200 U/mL, 15 min). (1) Control group, (2) Control serum group, (3) TNF-α group, (4) 5% DTD serum group, (5) 10% DTD serum group, (6) 20% DTD serum group, and (7) PFT-α group. (A) The expression of ICAM-1 and p53. (B) The expression of ICAM-1 and p21. The band intensities were assessed by scanning densitometry. Data were presented as means ± S.D. of three independent experiments. A one-way analysis of variance was used to compare the multiple group means followed by Newman–Keuls test (##p < 0.01, versus control group; *p < 0.05, **p < 0.01, versus TNF-α group).

Figure 2 The protein expression of ICAM-1, p53 and p21. Cells were pretreated with DTD serum for 6 h and then co-treated with 200 U/mL TNF-α for 15 min. Cells were extracted and protein levels were determined by Western blots. The cells were pretreated with DTD serum (5, 10, and 20%, 6 h), PFT-α (25 μM, 30 min) and then co-treated with TNF-α. (1) Control group, (2) control serum group, (3) TNF-α group, (4) 5% DTD serum group, (5) 10% DTD serum group, (6) 20% DTD serum group, (7) PFT-α group. (A) The expression of ICAM-1 and p53. (B) The expression of ICAM-1 and p21. The band intensities were assessed by scanning densitometry. Data are presented as means ± S.D. of three independent experiments. A one-way analysis of variance was used to compare the multiple group means followed by Newman–Keuls test (##p < 0.01, versus control group; *p < 0.05, **p < 0.01, versus TNF-α group).