Figures & data
Figure 1. Chemical structure of labd-8(17)-en-15-oic acid.
Figure 2. Maximal changes in mean aortic pressure (MAP) (A) and heart rate (HR) (B) elicited by intravenous (i.v.) injections of increasing bolus doses (1–10 mg/kg) of labd-8(17)-en-15-oic acid (labd-8) in conscious SHRs (black columns) and WKY (white columns) rats. Values are means of changes expressed as a percentage of baseline values. Vertical bars indicate S.E.M. (5–6 rats per group). *p < 0.05 by Dunnett's test with respect to basal values.
Figure 3. Maximal changes in mean aortic pressure (MAP) (A) and heart rate (HR) (B) elicited by intravenous (i.v.) injections of increasing bolus doses (1–10 mg/kg) of labd-8(17)-en-15-oic acid (labd-8) in conscious SHRs subjected to i.v. pretreatment with vehicle (Veh, 1 mL/kg), propranolol (Prop, 2 mg/kg), methylatropine (MA, 1 mg/kg), or l-NAME (20 mg/kg). The effect of pretreatment with i.v. hexamethonium was also investigated on the maximal changes in MAP (C) and HR (D) elicited by labd-8 (1–10 mg/kg). Values are means of changes expressed as a percentage of baseline values. Vertical bars indicate S.E.M. (5–6 rats per group). *p < 0.05 by Dunnett's test with respect to basal values. #p < 0.01 by two-way ANOVA with respect to vehicle-pretreated SHRs.
Figure 4. Effects of increasing concentrations (1–1000 μg/mL) of labd-8(17)-en-15-oic acid (labd-8) on the contraction induced by KCl (60 mM) in isolated thoracic aortic preparations with functional endothelium from WYK rats (n = 5) and SHRs (n = 8). The values for the pre-contraction induced by KCl in rings from WKY and SHRs were 2.35 ± 0.17 and 1.96 ± 0.16 g, respectively. No significant difference was observed between the two values. Vertical bars indicate S.E.M. *p < 0.05 by Dunnett's test with respect to basal values.
Figure 5. Effects of increasing concentrations (0.00004–4.1 µg/mL) of the positive reference drug forskolin on the contraction induced by KCl (60 mM) in isolated thoracic aortic preparations with functional endothelium from WYK rats (n = 4) and SHRs (n = 3). Vertical bars indicate S.E.M. *p < 0.05 by Dunnett's test with respect to basal values.
