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Original Article

A new ester of fatty acid from a methanol extract of the whole plant of Amaranthus spinosus and its α-glucosidase inhibitory activity

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Pages 600-604 | Received 03 Mar 2014, Accepted 12 Jun 2014, Published online: 23 Oct 2014

Figures & data

Figure 1. Structure of (14E, 18E, 22E, 26E)-methyl nonacosa-14, 18, 22, 26 tetraenoate.

Figure 1. Structure of (14E, 18E, 22E, 26E)-methyl nonacosa-14, 18, 22, 26 tetraenoate.

Figure 2. HPLC chromatograms of two isolated compounds from chloroform fraction of methanol extract of Amaranthus spinosus.

Figure 2. HPLC chromatograms of two isolated compounds from chloroform fraction of methanol extract of Amaranthus spinosus.

Figure 3. HPLC chromatograms of the purified fatty acid from Amaranthus spinosus.

Figure 3. HPLC chromatograms of the purified fatty acid from Amaranthus spinosus.

Figure 4. Inhibitory effect of methanol extract and fatty acid on α-glucosidase (0.5 U/mL, using substrate p-nitrophenyl α-d-glucopyranoside 0.5 mm) determined in the concentration range from 1 to 10 µM/mL.

Figure 4. Inhibitory effect of methanol extract and fatty acid on α-glucosidase (0.5 U/mL, using substrate p-nitrophenyl α-d-glucopyranoside 0.5 mm) determined in the concentration range from 1 to 10 µM/mL.

Figure 5. Kinetic analysis of α-glucosidase inhibition by novel fatty acid and methanol extract of A. spinosus. The Lineweaver–Burk plot of hydrolysis of p-nitrophenyl α-d-glucopyranoside (10, 15, 20, and 25 mM) by α-glucosidase (0.5 U/mL) in the presence and absence of isolated fatty acid and methanol extract.

Figure 5. Kinetic analysis of α-glucosidase inhibition by novel fatty acid and methanol extract of A. spinosus. The Lineweaver–Burk plot of hydrolysis of p-nitrophenyl α-d-glucopyranoside (10, 15, 20, and 25 mM) by α-glucosidase (0.5 U/mL) in the presence and absence of isolated fatty acid and methanol extract.

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