In vivo hair growth-stimulating effect of medicinal plant extract on BALB/c nude mice

Figures & data

Figure 1. (a) Comparative pattern of hair growth in different treatment groups. Mice were treated with different extracts with vehicle, Control (A), Minoxidil (B), P. multiflorum (C) and C. zawadaskii (D) topically applied for two hair growth generations. (b) Hair existing area. (c) Hair length of different treatment groups on first and second hair existing phases. Values are shown as mean ± SD. p ≤ 0.05, **p ≤ 0.001 compared with the corresponding control.

Figure 2. Gross phenotype (a–d) and the highlighted area are enlarged in (a′–d′) to show observation of dorsal skin. Skin specimen of dorsal skin of mice (a″–d″) haematoxylin and eosin (H&E) stained histology. Arrows indicate distorted hair follicles (b″, c″) and the straight hair shaft emerging through the epidermis (d″), Scale bar 500 µm. BrdU-labeled S-phase cells in the anagen hair bulb and outer root sheath (b″′, c″′ and d″′, brown color), scale bar 50 µm.

Figure 3. Transverse section of dorsal skin specimen of different treatment groups. Control (a), Minoxidil (b), P. multiflorum (c), C. zawadaskii (d) stained with haematoxylin and eosin (H&E). Dotted lines indicate the junction of dermis and subcutis. Scale bar 500 µm.

Table 1. Effect of different treatments on the number of hair follicles.

	Number of hair follicle (HF)
Group	In subcutis	Total HF
Control	12.33 ± 2.19	25 ± 2.83
Minoxidil	30.33 ± 2.84**	40.83 ± 3.8*
PM	18.43 ± 2.09	28 ± 2.72
CZ	39 ± 2.59**	58.67 ± 3.72**

The number of HFs in subcutis with respect to the total number of HFs (per field) (n = 10) are expressed as the mean ± SD.

*p ≤ 0.05, **p ≤ 0.001 compared with the corresponding control.