Figures & data
Table 1. Partition coefficients (K) for bergapten in reversed-phase systems.
Figure 1. HPLC chromatogram of the crude extract from Heracleum leskovii (A) together with the isolated compound and its UV-DAD spectrum (B) (λ = 254 nm).
Figure 2. Myorelaxant effect of bergapten dissolved in 0.5% DMSO on the spontaneous motor activity of isolated jejunum strips. The results are expressed as % of the relaxation caused by isoproterenol applied in the reference dose of 0.1 μM. The relaxation provoked by isoproterenol in the reference dose is expressed as 100%. The results are expressed as a mean from 7 to 8 independent experiments (±SD). *p ≤ 0.05 versus Isop, ▴p ≤ 0.05 versus DMSO, 0.5%.
Figure 3. Contractile effect of bergapten dissolved in 0.5% DMSO on the spontaneous motor activity of isolated jejunum strips. The results are expressed as % of the contraction caused by acetylcholine applied at the reference dose of 1 μM. The contraction provoked by acetylcholine at the reference dose is expressed as 100%. The results are expressed as a mean from 7 to 8 independent experiments (±SD). *p ≤ 0.05 versus ACh, ▴p ≤ 0.05 versus DMSO, 0.5%.
Figure 4. Representative recordings of the response of the isolated jejunum strips to bergapten (10 μM). (A) An example of a myorelaxant response. (B) An example of a contractile response.
Figure 5. The effect of the application of bergapten (10 μM) dissolved in DMSO (0.5%) on the amplitude of the spontaneous motor activity of rat isolated jejunum strips. (A) The amplitude of the spontaneous motor activity of jejunum strips which reacted through relaxation to a bergapten (10 μM) application. (A′) The amplitude of the spontaneous motor activity of jejunum strips which reacted through contraction to a bergapten (10 μM) application. The results are expressed as % of the amplitude of the spontaneous motor activity of isolated jejunum strips registered after DMSO (0.5%) application. The amplitude registered after DMSO (0.5%) administration is expressed as 100%. The results are expressed as a mean from 7 to 8 independent experiments (±SD). *p ≤ 0.05 versus DMSO, 0.5%.
