Endophytic bacteria from Pinellia ternata, a new source of purine alkaloids and bacterial manure

Figures & data

Figure 1. Guanosine and inosine content of fermentation broths containing endophytic bacteria isolated from P. ternata. Alkaloid content was quantified by UV absorption at 248 nm for inosine and 254 nm for guanosine, but only absorption at 254 nm is shown. (1) Standard solution; (2) Bacillus thuringiensis; (3) Aranicola proteolyticus; (4) Bacillus cereus; (5) Serratia liquefaciens; and (6) Bacillus licheniformis. Peak a: inosine; Peak b: guanosine.

Table 1. Alkaloid production by endophytic bacteria isolated from P. ternata^a.

Fermentation broth	Guanosine/(mg/L)	Inosine/(mg/L)
Control	0.0	0.0
Bacillus cereus	2.360 ± 0.051	2.701 ± 0.064
Aranicola proteolyticus	3.652 ± 0.047	3.058 ± 0.033
Serratia liquefaciens	5.143 ± 0.073	3.886 ± 0.040
Bacillus thuringiensis	8.082 ± 0.046	2.858 ± 0.052
Bacillus licheniformis	8.718 ± 0.059	3.709 ± 0.044

^aData are expressed as means ± standard deviations from three independent experiments, each with three replicates.

Table 2. Effect of Serratia liquefaciens and Bacillus licheniformis on PLB proliferation and differentiation.

	Serratia liquefaciens		Bacillus licheniformis
Treatments	GI^a	DR^b	GI	DR
Live bacteria control	3.11	50	3.23	50
0.5%	4.91	98	3.35	29
1.5%	5.31	93	3.57	13

^aGI (growth index) = final PLB fresh weight divided by initial PLB fresh weight.

^bDR (differentiation rate) = number of differentiated PLBs divided by the total number of PLBs.