The protective effect of atractylenolide I on systemic inflammation in the mouse model of sepsis created by cecal ligation and puncture

Figures & data

Figure 1. Effect of AT-I on survival of mice after CLP surgery. Mice were divided into five groups (n = 25): sham, control (0 mg/kg), AT-I (10, 20, and 40 mg/kg, ip). The mice were observed for 7 days. Asterisks indicated significant difference from control group, *p < 0.05, **p < 0.01.

Figure 2. Effect of AT-I on body temperature of CLP mice. Mice were divided into 5 groups (n = 25): sham, control (0 mg/kg), AT-I (10, 20, and 40 mg/kg, ip). The mice were observed for 72 hours. Asterisks indicated significant difference from control group, *p < 0.05, **p < 0.01.

Figure 3. Effects of AT-I on LPS and WBC level of mice after CLP surgery. Mice were divided into five groups (n = 15): sham, control (0 mg/kg), AT-I (10, 20, and 40 mg/kg, ip). Asterisks indicated significant difference from control group, *p < 0.05, **p < 0.01.

Table 1. Effect of AT-I on TNF-α, IL-1β and IL-6 of mice after CLP surgery.

	TNF-α (pg/mL)	IL-6 (pg/mL)	IL-1β (pg/mL)
Sham	6.79 ± 2.34**	19.97 ± 3.54**	16.53 ± 3.31**
0 mg/kg	108.96 ± 8.74	974.38 ± 94.35	538.61 ± 74.3
10 mg/kg	83.42 ± 6.32*	752.89 ± 76.72*	493.27 ± 66.36
20 mg/kg	71.07 ± 5.49**	604.45 ± 75.04**	371.73 ± 65.52*
40 mg/kg	54.72 ± 5.41**	479.42 ± 56.02**	318.92 ± 53.13**

Mice were divided into five groups (n = 15): sham, control (0 mg/kg), AT-I (10, 20, and 40 mg/kg, ip). Asterisks indicated significant difference from control group, *p < 0.05, **p < 0.01.

Table 2. Effect of AT-I on liver and kidney functions of mice after CLP surgery.

	Liver function		Kidney function
	ALT (U/L)	AST (U/L)	Cre (μmoL/L)	BUN (mmoL/L)
Sham	41.98 ± 7.91**	58.99 ± 9.62**	40.58 ± 3.67**	7.12 ± 1.16**
0 mg/kg	253.96 ± 34.64	475.63 ± 58.53	123.96 ± 14.72	21.79 ± 2.81
10 mg/kg	172.31 ± 21.65*	359.85 ± 51.28*	116.31 ± 12.73	16.45 ± 2.48*
20 mg/kg	139.54 ± 15.97**	228.61 ± 43.85**	89.54 ± 12.92*	14.31 ± 1.75**
40 mg/kg	84.29 ± 13.98**	126.42 ± 29.41**	74.29 ± 11.89**	10.62 ± 1.33**

Mice were divided into five groups (n = 15): sham, control (0 mg/kg), AT-I (10, 20, and 40 mg/kg, ip). Asterisks indicated significant difference from control group, *p < 0.05, **p < 0.01.