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Original Article

Gene expression profiling of DMU-212-induced apoptosis and anti-angiogenesis in vascular endothelial cells

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Pages 660-666 | Received 18 Apr 2015, Accepted 07 Jul 2015, Published online: 01 Oct 2015

Figures & data

Figure 1. The chemical structure of trans-3,4,5,4′-tetramethoxystilbene (DMU-212). DMU-212 has the molecular formula C18H20O4 and a molecular weight of 300.3490 g/mol.

Figure 1. The chemical structure of trans-3,4,5,4′-tetramethoxystilbene (DMU-212). DMU-212 has the molecular formula C18H20O4 and a molecular weight of 300.3490 g/mol.

Table 1. Genes whose average expression differed by greater than 2-fold are grouped by functional category: labeled cRNA prepared from HUVECs at 6 h after infection with DMU-212 use Affymetrix Human GeneChip® Gene 1.0 ST Arrays.

Figure 2. Verification of microarray analysis by qRT-PCR. Histogram showed the mRNA levels of genes as indicated. Data are expressed as the mean (±SE) of three independent experiments. *p < 0.05, **p < 0.01 versus control.

Figure 2. Verification of microarray analysis by qRT-PCR. Histogram showed the mRNA levels of genes as indicated. Data are expressed as the mean (±SE) of three independent experiments. *p < 0.05, **p < 0.01 versus control.

Figure 3. Biological process classification of the differential expressed genes using the GENEONTOLOGY (The Gene Ontology Consortium, Cambridge, UK) and PANTHER (Evolutionary Systems Biology Group, Menlo Park, CA) database.

Figure 3. Biological process classification of the differential expressed genes using the GENEONTOLOGY (The Gene Ontology Consortium, Cambridge, UK) and PANTHER (Evolutionary Systems Biology Group, Menlo Park, CA) database.

Figure 4. Network extension of the comprehensive signaling pathway collated by the differentially expressed genes that were regulated by DMU-212.

Figure 4. Network extension of the comprehensive signaling pathway collated by the differentially expressed genes that were regulated by DMU-212.

Table 2. Pathway identification analysis using KOBAS.

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