Cytotoxic activity of physodic acid and acetone extract from Hypogymnia physodes against breast cancer cell lines

Figures & data

Figure 1. Structure of physodic acid.

Table 1. Cytotoxic effect of physodic acid and acetone extract obtained from H. physodes against selected human cancer cell lines.

	Cell viability (%)^a
Sample	MDA-MB-231	MCF-7	T47D
Physodic acid^b	0.0 ± 1.40	0.0 ± 2.74	0.0 ± 0.79
Acetone extract^c	47.6 ± 11.72	52.7 ± 4.23	36.5 ± 4.53

a Values are expressed as mean n = 6 replications ± SD; measured after 72 h of exposure the cells to the test compounds; MTT assay.

b Concentration: 100 μM.

c Concentration: 100 μg/mL.

Table 2. In vitro cytotoxic activities of acetone extract from H. physodes.

Cell line	MTT^a (IC50 μg/mL)
MDA-MB-231	46.2 ± 10.2
MCF-7	110.4 ± 21.3
T47D	60.0 ± 11.3

a Values are expressed as mean n = 6 replications; measured after 72 h of exposure the cells to the test compounds.

Table 3. In vitro cytotoxic activities of physodic acid.

	IC50 values (μM)^a
Test	MDA-MB-231	MCF-7	T47D	MCF-10A
MTT	93.9 ± 10.2	72.4 ± 7.9	75.4 ± 12.6	>100
NRU	68.8 ± 14.3	46.0 ± 11.3	53.9 ± 6.9	>100
CV	71.9 ± 14.8	48.3 ± 12.6	49.5 ± 9.4	>100

a Values are expressed as mean n = 6 replications; measured after 72 h of exposure the cells to the test compounds.

Table 4. Antioxidant activity of acetone extract from H. physodes.

Sample	DPPH (IC50 μg/mL)	FRAP (μg VCE/mL of samples)^c
H. physodes extract^a	21.49 ± 0.81	7.97 ± 0.16
Vitamin C^b	4.04 ± 0.02	–
BHA^b	4.14 ± 0.04	–

Note: Values are expressed as mean.

a n = 12.

b n = 6 replications ± SD.

c Concentration of tested extract 50 μg/mL.