Antioxidative iridoid glycosides from the sky flower (Duranta repens Linn)

Figures & data

Table 1.  Antioxidative activity of the solvent fractions.

Sample	IC50 [µg/mL] ^a)
	^•OH^b)	Total ROS^c)	ONOO^−d)
CHCl3	46.38 ± 0.09	> 400	63.27 ± 0.07
EtOAc	16.21 ± 0.05	67.30 ± 0.09	21.51 ± 0.03
n-BuOH	31.43 ± 0.03	> 300	51.1 ± 0.09
H2O	73.49 ± 0.04	> 400	91.07 ± 0.05
Trolox ^e)	5.79 ± 0.06	35.30 ± 0.05	–
DL-Penicillamine ^f)	–	–	1.07 ± 0.04

^aValues of OH, total ROS, and ONOO⁻ are expressed as the mean ± standard error of triplicate experiments.

^bInhibitory activity of hydroxyl radical generation in 1.0 mM H₂O₂ and 0.2 mM FeSO₄.

^cInhibitory activity of total ROS generation in kidney postmicrosomal fraction.

^dInhibitory activity of authentic peroxynitrite.

^eTrolox was used as a positive control.

^fDL-Penicillamine was used as a positive control.

Figure 1.  Structures of known compounds 1–6.

Table 2.  Antioxidative activity of compounds 1–6.

Compound	IC50 [µM] ^a)			DPPH IC50 ± SEM^a) [mM]
	^•OH^b)	Total ROS^c)	ONOO^− d)
1	7.23 ± 0.05	63.72 ± 0.07	8.9 ± 0.04	0.595 ± 0.02
2	4.07 ± 0.03	43.30 ± 0.05	3.39 ± 0.02	0.481 ± 0.06
3	10.13 ± 0.06	47.51 ± 0.07	14.17 ± 0.05	0.621 ± 0.07
4	10.67 ± 0.02	90.21 ± 0.03	16.91 ± 0.09	0.662 ± 0.05
5	17.21 ± 0.05	97.37 ± 0.05	18.94 ± 0.07	0.719 ± 0.03
6	9.18 ± 0.06	87.19 ± 0.06	11.09 ± 0.08	0.607 ± 0.10
Trolox ^e)	2.85 ± 0.07	30.15 ± 0.17	–	–
DL-Penicillamine ^f)	–	–	1.09 ± 0.07	–
3-t-butyl-4-hydroxyanisole (BHA)^g)	–	–	–	0.049 ± 0.05

^aValues of ^•OH, total ROS, ONOO⁻ and DPPH are expressed as the mean ± standard error of triplicate experiments.

^bInhibitory activity of hydroxyl radical generation in 1 mM H₂O₂ and 0.2 mM FeSO₄.

^cInhibitory activity of total ROS generation in kidney postmicrosomal fraction.

^dInhibitory activity of authentic peroxynitrite.

^eTrolox was used as a positive control.

^fDL-Penicillamine was used as a positive control.

^gpositive control used in DPPH assays.