Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity

Ramazan DemirdagAtaturk University, Science Faculty, Department of Chemistry, Erzurum, Turkey

Emrah YerlikayaAtaturk University, Science Faculty, Department of Chemistry, Erzurum, Turkey

Omer Irfan KufreviogluAtaturk University, Science Faculty, Department of Chemistry, Erzurum, TurkeyCorrespondence[email protected]

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Table 1. Summary of purification procedure for sheep liver CA II.

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Figure 1. SDS-PAGE analysis of purified sheep CA II. Lane (1) sheep- CA II. Lane (2) standard proteins (E. coli b-galactosidase (116 kDa), rabbit phosphorylase B (97.4 kDa), bovine serum albumin (66 kDa), chicken ovalbumin (45 kDa) and bovine carbonic anhydrase (29 kDa)).

Table 2. K_i values and inhibition types for four metal ions of SCA II.

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Table 3. IC₅₀ values of inhibitors for SCA-II hydratase activity.

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Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity

Table 1. Summary of purification procedure for sheep liver CA II.

Table 2. K_i values and inhibition types for four metal ions of SCA II.

Table 3. IC₅₀ values of inhibitors for SCA-II hydratase activity.

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Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity

Figures & data

Table 1. Summary of purification procedure for sheep liver CA II.

Table 2. Ki values and inhibition types for four metal ions of SCA II.

Table 3. IC50 values of inhibitors for SCA-II hydratase activity.

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Table 2. K_i values and inhibition types for four metal ions of SCA II.

Table 3. IC₅₀ values of inhibitors for SCA-II hydratase activity.