Inhibition of the alpha- and beta-carbonic anhydrases from the gastric pathogen Helycobacter pylori with anions

Figures & data

Table 1.  Inhibition constants of anion inhibitors against α-/β-CAs from mammals (hCA I, and II, human isoforms) and bacteria: PCA (from S. pneumoniae), and hpαCA/hpβCA (from H. pylori) for the CO₂ hydration reaction, at 20 °C²².

Inhibitor^a	KI [mM]^b			hpαCA^c	hpβCA^c
	hCA I^d	hCA II^d	PCA^e
F^−	> 300	>300	0.85	4.08	0.67
Cl^−	6	200	0.052	2.70	0.56
Br^−	4	63	0.046	2.41	0.38
I^−	0.3	26	0.054	6.05	0.63
CNO^−	0.0007	0.03	0.098	0.60	0.37
SCN^−	0.2	1.60	0.38	4.10	0.68
CN^−	0.0005	0.02	0.041	0.76	0.54
N3^−	0.0012	1.51	0.35	0.83	0.80
HCO3^−	12	85	0.33	0.75	0.50
CO3^2−	15	73	0.53	0.66	0.42
NO3^−	7	35	0.39	0.81	0.78
NO2^−	8.4	63	0.66	0.93	0.67
HS^−	0.0006	0.04	0.35	0.69	0.58
HSO3^−	18	89	0.57	0.99	0.63
SnO3^2−	0.57	0.83	0.066	0.55	0.48
SeO4^2−	118	112	0.044	0.72	0.65
TeO4^2−	0.66	0.92	0.049	0.34	0.45
OsO5^2−	0.92	0.95	0.060	0.48	0.89
S2O7^2−	0.99	0.97	0.048	0.71	0.61
P2O7^4−	25.77	48.50	0.45	0.66	0.75
V2O7^4−	0.54	0.57	0.038	0.27	0.18
B4O7^2−	0.64	0.95	0.32	0.56	0.68
ReO4^−	0.110	0.75	0.039	0.88	0.82
RuO4^−	0.101	0.69	0.036	0.36	1.10
S2O8^2−	0.107	0.084	0.046	0.92	0.93
SeCN^−	0.085	0.086	0.022	0.73	0.97
CS3^2−	0.0087	0.0088	0.021	0.38	0.21
Et2NCS2^−	0.79	3.10	0.61	0.0049	0.0074
SO4^2−	63	>200	4.15	0.82	0.57
ClO4^−	>200	>200	>200	10.1	6.50
BF4^−	>200	>200	>200	>200	>200
FSO3^−	0.79	0.46	0.060	0.91	0.75
NH(SO3)2^2−	0.31	0.76	28.1	0.54	0.70
H2NSO2NH2	0.31	1.13	4.25	0.073	0.072
H2NSO3H	0.021	0.39	6.68	0.080	0.094
Ph-B(OH)2	58.6	23.1	6.47	0.097	0.073
Ph-AsO3H2	31.7	49.2	5.86	0.44	0.092

^aAs sodium salt.

^bErrors were in the range of 3%–5 % of the reported values, from three different assays;

^cThis work.

^d,eFrom references^9,25,26.

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