Inhibition of fatty acid synthase by ginkgolic acids from the leaves of Ginkgo biloba and their cytotoxic activity

Figures & data

Table 1.  Inhibitory activity of ginkgolic acids 1–3 isolated from G. biloba against FAS.

Compound	FAS inhibitory activity
	IC50 (µM)*
Ginkgolic acid C15:1 (1)	17.1 ± 1.5
Ginkgolic acid C17:2 (2)	9.2 ± 1.0
Ginkgolic acid C17:1 (3)	10.5 ± 1.3
Luteolin^†	62.5 ± 4.0
Cerulenin^†	13.0 ± 0.9

*IC₅₀values were determined by regression analyses and expressed as means of three replicates ± standard deviation.

^†Positive control.

FAS, fatty acid synthase.

Table 2.  Cytotoxic activity of ginkgolic acids 1–3 isolated from G. biloba (IC50 µM)*.

Compound	MCF-7	A549	HL-60
Ginkgolic acid C15:1 (1)	146.3	66.8	5.0
Ginkgolic acid C17:2 (2)	108.2	56.4	4.0
Ginkgolic acid C17:1 (3)	93.2	50.5	4.2
Adriamycin^†	3.5	1.8	0.8
Cyclophosphamide monohydrate^†	3792.5	NT	NT

*IC₅₀values were determined by regression analyses and expressed as means of three replicates.

^†Positive controls.

NT, not tested.

Figure 1.  Structures of ginkgolic acids 1–3 isolated from the leaves of G. biloba.