Quiescent stem cells in intestinal homeostasis and cancer

Figures & data

Table 1. Overview of quiescent stem cells characterized to date.

Tissue type	Markers	Reference
cornea	3H-thymidine label retention	Cotsarelis et al., 1989
hair follicle	Label retention Cytokeratin 15 Cytokeratin 15 H2B-GFP label retention	Cotsarelis et al., 1990 Lyle et al., 1998 Lyle et al., 1999 Tumbar et al., 2004
hematopoietic system	H2B-GFP label retention H2B-GFP label retention	Wilson et al., 2008 Foudi et al., 2009
mammary gland	BrdU label retention, Sca-1 CD29hiCD24+ PKH26 label retention	Welm et al., 2002 Shackleton et al., 2006 Pece et al., 2010
forebrain	3H-thymidine label retention	Morshead et el., 1994
pancreas	label retention	Teng et al., 2007
prostate	BrdU label retention	Tsuijmura et al., 2002
small intestine	BrdU label retention Msi-1 Msi-1 Dcamkl1 Bmi1 mTert mTert H2B-GFP label retention	Potten et al., 1998 Potten et al., 2003 Nishimura et al., 2003 May et al., 2008 and 2009 Sangiorgi & Capecchi, 2008 Breault et al., 2008 Montgomery et al., 2011 Roth et al., 2011
stomach	Villin	Qiao et al., 2007

Figure 1. Tet-inducible strategies in pulse-chase experiments to selectively label and prospectively isolate quiescent (stem) cells by H2B-GFP retention.

Figure 2. Hypothetical model for the quiescent stem cell origin of inflammation-associated cancers.

Table 2. Overview of quiescent cancer stem cells characterized to date.

Cancer type	Method used	Reference
Ovarian adenocarcinoma	PKH26, PKH67 proliferation rate	Kusumbe & Bapat, 2009 Gao et al., 2010
Pancreas adenocarcinoma	DiI (C59H97ClN2O4)	Dembinski & Krauss, 2009
Melanoma	PKH26	Roesch et al., 2010
Breast cancer	PKH26	Pece et al., 2010
Hepatocellular carcinoma	Hoechst 33342 and PY	Haraguchi et al., 2010
AML (acute myelogenous leukaemia)	cell cycle analysis	Saito et al., 2010

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