Figures & data
Figure 1. The workflow of a typical proteomic experiment as performed in this study.
Figure 2. (A) Total ion chromatogram of nano-LC/MS/MS analysis of tryptic peptides obtained from a fraction of DON-treated cell line extract. (B) Tandem mass spectra of the peak at the retention time 26.04 min.
Figure 3. (A) Illustrates the number of proteins identified in both the DON-exposed sample and the control of Experiment I, as well as the proteins detected only either in the DON-treated sample or in the control. To obtain the total proteins identified in Experiment I, proteins present in the control and in the sample were combined. (B) Indicates the number of proteins identified that are common to both sample and control of Experiment II, and the proteins detected either in the sample or in the control. The total proteins identified in Experiment II were obtained by combining proteins present in the sample and in the control.
Table 1. Identified proteins affected by the exposure of EL4 cells to deoxynivalenol (DON).a
Table 2. List of the proteins detected only in DON-exposed cells.
Table 3. List of the up- and down-regulated proteins with gene symbols, peptide ratios* and the pathways implicated.
Figure 4. A scheme illustrating the oxidative protein folding pathway in the endoplasmic reticulum (ER) (adapted from Tu and Weissman, Citation2004). DON induced the oxido-reductants, protein disulfide isomerase (PDI), and endoplasmic oxido-reductin-1 (ERO1-l) which catalyze this pathway. An increase in protein cargo (synthesis) or increase in mis-folded proteins (e.g., under oxidative stress) stimulates this pathway.
