In vitro myelotoxic effects of cypermethrin and mancozeb on human hematopoietic progenitor cells

Figures & data

Figure 1. Observed colonies in the methylcellulose-based clonogenic assay – after 14 days. (A) CFU-E (200×): An isolated single colony containing ≈8–200 erythroblasts. (B) BFU-E (20×): > 200 erythroblasts in a single or multiple clusters. (C–F) CFU-GM (20×): An aggregate containing at least ≥50 granulocytes (CFU-G), macrophages (CFU-M), or cells of both lineages. (G, H) CFU-GEMM (100×): > 500 cells containing erythroblasts and cells of at least two other recognizable lineages.

Table 1. IC₅₀ (µM) values with erythropoietic, granulopoietic, and primitive progenitor colonies.

	Erythroid			Granulopoietic	Primitive progenitor
Test agent	CFU-E	BFU-E	TOTAL-E	CFU-GM	CFU-GEMM
Cypermethrin	6.4 ± 0.3	11.4 ± 0.5	8.7 ± 0.2	19.2 ± 0.9	6.2 ± 0.3
Mancozeb	5.3 ± 0.2	6.6 ± 0.2	6.2 ± 0.2	8.1 ± 0.2	2.8 ± 0.3
Benzo(a)pyrene	4.5 ± 0.2	7.7 ± 0.2	6.6 ± 0.1	11.1 ± 0.4	4.0 ± 0.3
Urethane	674.8 ± 21.8	879.2 ± 24.8	801.2 ± 15.0	877.2 ± 24.0	473.3 ± 12.4

IC₅₀ values (mean ± SE) were calculated using the formula of Reed & Muench (1938) and was based on three experiments carried out in triplicate (independently) using blood samples obtained from three individual donors for each compound.

Reed, L. J., and Muench, H. A. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27:493–497

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