Comparative human and mouse antibody responses against tetanus toxin at clonal level

Figures & data

Table 1. Representative screening results of mouse TeNT-specific hybridoma clones. (Results represent optical density (OD) values obtained by ELISA at 450 nm.).

mAb	Toxin	Toxoid
1F3E3	>3.0	>3.0
1F2C2	>3.0	>3.0
1F1E12	>3.0	>3.0
1F2C8	>3.0	>3.0
1F3B3	0.598	0.915
1F3C3	0.687	1.915
2F4C7	>3.0	2.493
2C9B6	>3.0	1.368
5E6B10	>3.0	>3.0
1E4D5	>3.0	>3.0
1G9F5	>3.0	2.416
1F6G7	>3.0	2.217
3B3D9	2.517	2.505
3E3F12	>3.0	>3.0
1F2B3	0.783	1.303
1F2E8	0.698	0.511
1F2G10	1.192	0.641
Blank	0.036	0.084

Table 2. Reactivity of human and mouse monoclonal antibodies with different fragments of tetanus toxin.

Species	Number of mAb	Toxoid	Toxin	FC	HCC	LC	FC + LC	None
Mouse	22	22	22	7	2	7	3	5
Human	50	50	50	34*	26**	10	2	4

FC, fragment C; LC, light chain; H_CC, C-terminal of fragment C; None, Specific mAb reacting with none of tested tetanus toxin fragments.

*, **Significant differences at p = 0.009 and 0.0004, respectively.

Figure 1. In vitro inhibitory activity of the mouse monoclonal antibodies on TeNT binding to GT1b. Individual clones are listed along the right side of the figure.

Table 3. Toxin neutralization activity of human and mouse anti-TeNT mAb as determined by GT1b binding inhibition assay.

Species	Neutralizing mAb	FC	HCC	LC	FC + LC	None
Mouse	6	6	1	0	0	0
Human	8	6	6*	0	1	1

See footnote to Table 2 for description of TeNT fragments.

*Significant difference at p = 0.015.

Table 4. Representative data obtained for a number of murine mAb and human LCLs specific for tetanus toxin. (Results represent optical density (OD) values obtained by ELISA at 450 nm.).

mAb/LCL	FC	HCC	LC	Neutralizing activity (%)
1F3E3	1.682	1.257	0.104	93.40
1F2C2	2.580	0.066	0.085	87.40
1F3B3	0.208	0.013	1.003	2.00
1E4D5	0.907	0.699	0.060	0.0
1G9F5	0.044	0.0 17	0.065	0.0
Blank	0.041	0.059	0.061	–
TTX1	1.691	1.659	0.039	92.3
TTX12	>3.0	1.550	0.032	73.1
TTX18	0.842	0.051	0.021	0.0
TTX24	0.084	0.062	0.085	0.0
TTX26	0.079	0.055	2.484	0.0
Blank	0.025	0.042	0.090	–

See footnote to Table 2 for description of TeNT fragments.