Association between nitric oxide and 8-hydroxydeoxyguanosine levels in semen of diabetic men

Figures & data

Table 1. Semen characteristics and biochemical data of diabetic and non-diabetic men.

Parameters	Diabetic (n = 32)	Non-diabetic (n = 35)	p value
Semen parameters:
Volume (ml)	3.22 ± 1.47	3.64 ± 1.30	0.226
Sperm concentration (10^6/ml)	98.28 ± 54.76	87 ± 37.59	0. 326
Total sperm number (10^6)	326.44 ± 233.71	311.31 ± 134.27	0.744
^aMotility grade a + b + c (%)	63.84 ± 8.37	67.51 ± 5.73	0.137
Normal morphology (%)	33.84 ± 3.21	35.25 ± 3.82	0.108
Biochemical parameters:
Fasting blood glucose (mg/dl)	199.30 ± 50.60	83.60 ± 6.20	0.001
HbA1c (%)	7.60 ± 1.17	4.1 ± 0.42	0.001
Triglycerides (mg/dl)	116.44 ± 65.21	92.42 ± 28.12	0.061
Total cholesterol (mg/dl)	171.21 ± 27.80	161.43 ± 18.26	0.090
HDL-cholesterol (mg/dl)	38.03 ± 5.61	41.25 ± 10.97	0.081
LDL-cholesterol (mg/dl)	106.41 ± 25.68	100.89 ± 19.87	0.320

Results are presented as mean values ± standard deviation (SD).

^aGrade of sperm movement according to WHO [1999] criteria: a = rapid progressive, b = slow progressive, c = non-progressive.

Figure 1.  Seminal plasma levels of nitrite in diabetic and non-diabetic men. Nitrate/nitrite was quantified by colorimetric assay. The optical density was measured at 540 nm and the concentration was determined by standard curve. The * indicates significance at p < 0.01.

Figure 2.  Seminal plasma concentration of 8-OHdG in diabetic and non-diabetic men. 8-hydroxy-2-deoxyguanosine (8-OHdG) levels were measured in seminal plasma using a competitive ELISA. The * indicates significance at p < 0.05.

Figure 3.  Correlation between nitrite concentration and the concentration of 8-OHdG in the seminal plasma. A) diabetic and B) non-diabetic men. Association between variables was tested using linear regression analysis and Pearson's correlation coefficient.