Figures & data
Figure 1. Isolation of bovine blastocoel fluid. (A) A mixed population of blastocysts with arrested embryos (▪), expanded (○), and hatched blastocysts (•). Notice the empty zona pellucida (□). (B) Collection of expanded blastocysts with a clear blastocoel cavity chosen for micromanipulation. (C) A single blastocyst is fixed on a holding pipette with the inner cell mass (ICM) pointing towards the top of the holding pipette. (D) The micropipette is inserted in the blastocoel cavity. (E) Suction applied and the blastocoel is aspirated until the blastocyst collapses.
Figure 2. Venn diagrams over protein identifications in bovine blastocoel fluid and blastocyst cells with an overview of the proteins in each biological replicate. Figure not to scale.
Table 1. Proteins identified in the bovine blastocoel fluid.
Figure 3. The distribution of gene ontology (GO) terms of the proteins from the bovine blastocyst cells. A protein can appear in more than one category.
Table 2. Selected proteins from the bovine blastocyst cells with the gene ontology (GO) terms cell differentiation, cell proliferation, development, and reproduction.
Table 3. Selected proteins identified in both human and bovine blastocyst cells.