Molecular evidence that Langeronia macrocirra and Langeronia cf. parva (Trematoda: Pleurogenidae) parasites of anurans from Mexico are conspecific

Figures & data

Table I.  Host, locality, geographical coordinates, and GenBank accession numbers for cox1 and ITS2 sequences of analyzed samples.

			GenBank accession numbers
Host	Locality	Geographical coordinates	ITS2	cox1
L. macrocirra
L. vaillanti	Laguna Escondida, Veracruz	18°35″30.2″N, 95°05′15.6″W	HM853578	HM853564
	Rio Pizote, Guanacaste, Costa Rica	10°56′24″N, 85°24′46″W	HM853580	HM853568
L. berlandieri	La Selva Biological Station, Costa Rica	10°26′15.03″N, 84°0′1.18″W	–	HM853569
	El Petén: in front of Cerro Cahuí, Guatemala	16°59.82′N, 89°42.30′W	–	HM992938
R. marina	Lago de Catemaco, Veracruz	18°25′09.4″N, 95°06′51.5″W	HM853581	HM853565
	Coquimatlán, Colima	19°09′92″N, 103°49′56″W	HM853579, HM853582	HM853566
	Salto de Eyipantla, Veracruz	22°18′ N, 95°12′W	–	HM853567
L. cf parva
L. vaillanti	Laguna Escondida, Veracruz	18°35′N, 95°06′W	HM853571, HM853577	HM853557
	San José Independencia, Oaxaca	18°15′N, 96°13′W	HM853573, HM853575, HM853576	HM853559, HM853561, HM853563
	Chapultepec, Oaxaca	18°07′08″N, 96°50′2″W	HM853574	HM853560 HM853562
Lithobates sp. Colima^*	Coquimatlán, Colima	19°09′92″N, 103°49′56″W	HM853572	HM853558
P. chabaudi^†
L. vaillanti	Rio Pizote, Guanacaste, Costa Rica	10°56′24″N, 85°24′46″W	HM853570	HM853556

* This species is not yet described formally, but is recognized as Lithobates sp. Colima by Zaldívar-Riverón et al. (2004)

† Caballero and Caballero (1969) erected Brenesia with B. chabaudi from a species of Lithobathes; this species is typical of intestine of frogs. This genus has been subject to taxonomic controversy (Sullivan 1971). According to Lotz and Font (2008), Brenesia is a synonym of Pseudosonsinotrema within Pleurogenidae.

Figure 1.  Morphological comparison of two nominal species of identified Langeronia and SEM photomicrographs. (I) General morphology: L. macrocirra (A–C) and L. cf. parva (D–F). (A) Holotype, Lithobates sp. (locality unknown, CNHE 13885), Mexico. (B) R. marina Lago de Catemaco, Veracruz (CNHE 4875). (C) R. marina, Lago de Catemaco, Veracruz (CNHE 4875). (D) Paratype, Lithobates pipiens, Alburg, Vermont, USA (USNPC 70558) and L. vaillanti, Laguna Escondida, Veracruz (CNHE 4874). (E) L. vaillanti, Laguna Escondida, Veracruz (CNHE 4874). (F) L. vaillanti, Laguna Escondida, Veracruz (CNHE 4874). Reference bar: C = 0.5 mm and F = 0.3 mm. (II) SEM photomicrographs: L. macrocirra from Lago de Catemaco, Veracruz, R. marina: (A, B, F–I, K, and L). L. cf. parva from Laguna Escondida, Veracruz, L. vaillanti (C–E, and J).Whole worm without tuck (A and C) and with tuck (B and E). Details of the tuck (E and F). Ciliated dome-shaped papillae (type I) on ventral surface anterior part of the oral sucker (G). Excretory pore (H). Small non-ciliated dome-shaped papillae (type II) close to a papillae type I on ventral surface around the acetabulum (I). Egg rough elliptical and operculate (J). Tegumental spines allocated near to the acetabulum (K). Spines allocated near to the posterior end (L). Small papillae ciliated among spines (M).

Table II.  Pairwise genetic distances (%) among cox1 sequences of analyzed specimens.

		1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
1	PC	–
2	LP2	21.19	–
3	LM3	21.19	0	–
4	LM7	22.01	8.15	8.15	–
5	LM6	22.01	7.88	7.88	0.27	–
6	LM5	22.01	7.88	7.88	0.27	0	–
7	LM1	21.46	8.15	8.15	0.73	0.73	0.73	–
8	LP1	21.19	8.15	8.15	0.70	0.70	0.70	0.27	–
9	LM4	21.19	8.15	8.15	0.70	0.70	0.70	0.27	0	–
10	LP7	21.46	8.42	8.42	0.67	0.67	0.67	0.54	0.27	0.27	–
11	LM2	21.46	8.42	8.42	0.70	0.70	0.70	0.81	0.54	0.54	0.27	–
12	LP4	21.46	8.42	8.42	0.70	0.70	0.70	0.81	0.54	0.54	0.27	0	–
13	LP6	21.46	8.42	8.42	0.70	0.70	0.70	0.81	0.54	0.54	0.27	0	0	–
14	LP5	21.73	8.69	8.69	0.73	0.73	0.73	1.08	0.81	0.81	0.54	0.27	0.27	0.27	–
15	LP3	21.46	8.42	8.42	0.70	0.70	0.70	0.81	0.54	0.54	0.27	0	0	0	0.27	–

Notes: Distances are expressed as a percentage of nucleotide differences. LM1, L. macrocirra; LP, L. cf. parva; PC, P. chabaudi.

Figure 2.  Strict consensus tree of seven equally parsimonious trees obtained from maximum parsimony analysis using partial cox1 gene sequences. Bootstrap percentages are shown above internal nodes. Geographical origin is designated as follows: Colima: Coquimatlán (COL); Veracruz: Lago de Catemaco (CAT), Laguna Escondida (LE), Salto de Eyipantla (SE); Oaxaca: San José Independencia (SJI), Chapultepec (CH); Costa Rica: La Selva Biological Station (SELV), Rio Pizote, Guanacaste (GUAN); Guatemala: El Petén (PETEN). Hosts: L. vaillanti (Lv), L. berlandieri (Lber), Lithobates sp. Colima (Lcol), R. marina (Rm).

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