Evidence of new species of Haematoloechus (Platyhelminthes: Digenea) using partial cox1 sequences

Figures & data

Table I.  Hosts and collection localities of Haematoloechus species, and accession numbers of voucher specimens (in CNHE) and DNA sequences (in GenBank).

Haematoloechus species	Host species	Collection locality	Voucher number	Isolate (GenBank accession number)
H. coloradensis	Lithobates dunni	Michoacán, Mexico	3892	Ha19 (HQ141685)
H. complexus	L. pipiens	Georgia, USA		Ha58 (HQ141702)
H. cf. complexus	L. vaillanti	Veracruz, Mexico	4086	Ha15 (HQ141684)
				Ha51 (HQ141701)
H. cf. complexus	Ambystoma sp.	Puebla, Mexico		Ha86 (HQ141710)
H. danbrooksi	L. vaillanti	Veracruz, Mexico	4112, 4151	Ha49 (AY672116)
				Ha14 (HQ141683)
				Ha34 (HQ141693)
				Ha36 (HQ141694)
				Ha37 (HQ141695)
				Ha48 (HQ141699)
				Ha50 (HQ141700)
H. exoterorchis	Hoplobatrachus occipitalis	Sierra Leone	4662	Ha47 (HQ141698)
H. floedae	L. catesbeiana	California, USA	4664	Ha74 (HQ141709)
		Georgia, USA	4663	Ha75 (HQ141708)
				Ha70 (AY672119)
	L. cf. forreri	Guanacaste, Costa Rica	5065	Ha78 (AY672120)
				Ha79 (AY672121)
	Lithobates sp.	Chiapas, Mexico		Ha61 (HQ141703)
	L. vaillanti	Yucatán, Mexico	5067	Ha55 (AY672117)
				Ha57 (AY672118)
H. illimis	L. montezumae	Estado de Mexico	3381–3383	Ha20 (AY672122)
				Ha21 (HQ141686)
H. longiplexus	L. catesbeiana	Nebraska, USA	4664	Ha11 (HQ141682)
				Ha24 (HQ141688)
				Ha25 (HQ141689)
				Ha26 (HQ141690)
H. macrorchis	L. montezumae	Estado de Mexico	3394	Ha22 (HQ141687)
H. medioplexus	L. pipiens	Neligh, Nebraska, USA	4665	Ha17 (AY672123)
H. meridionalis	L. vaillanti	Guanacaste, Costa Rica	4199–4203	Ha9 (HQ141680)
				Ha10 (HQ141681)
		Veracruz, Mexico		Ha46 (HQ141697)
H. micrurus	H. occipitalis	Sierra Leone	4666	Ha44 (HQ141696)
H. parviplexus	L. catesbeiana	Nebraska, USA	4405	Ha16 (AY672124)
				Ha27 (HQ141691)
				Ha28 (HQ141692)
Haematoloechus sp.	L. psilonota	Jalisco, Mexico		Ha62 (HQ141704)
	Lithobates sp.	Oaxaca, Mexico		Ha64 (HQ141706)
Haematoloechus sp.	L. zweifeli	Oaxaca, Mexico		Ha65 (HQ141707)
Haematoloechus sp.	L. cf. forreri	Colima, Mexico		Ha63 (HQ141705)
H. varioplexus	L. blairi	Nebraska, USA		Ha12 (AY672125)

Figure 1.  One of six most parsimonious trees (tree length = 496; CI = 0.548) obtained from partial cox1 sequences of Haematoloechus spp. Numbers above the internode branches denote bootstrap support percentages. ★, potential new species.

Table II.  Percentage of sequence divergence among clades of Haematoloechus species recovered in this study.

1. H. varioplexus	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17
2. H. coloradensis	2.4	–
3. H. parviplexus	6.4–8.8	6.3–7.4	–
4. H. medioplexus	8.0	9.1	8.6–10.2	–
5. H. meridonalis	10.8–11.1	10.2–10.5	11.6–12.6	10.5–11.7	–
6. H. illimis	10.5	10.5–11.1	11.9–13.3	10.5	11.1–12.8	–
7. Haematoloechus sp. O	13.3	13.3	13.3–14.1	14.4	11.6–12.6	11.4–11.9	–
8. H. floedae	7.2–8.6	7.2–8.6	8.9–10.6	9.4–11.3	8.3–10.8	8.3–10.3	9.9–11.9	–
9. H. cf. complexus V	13.8–14.1	14.9–15.2	15.7–17.2	14.7–14.9	14.5–14.9	15.8–16.1	16.6–16.8	12.7–14.8	–
10. Haematoloechus sp. C	13.8	14.7	14.4–15.2	14.1	14.1–14.5	15.2	16.3	12.2–13.6	9.7–9.9	–
11. H. cf. complexus P	14.9	15.2	14.4–15.2	13.8	15.5–15.8	15.2–15.5	12.2	12.3–13.6	10.5–10.8	12.2	–
12. Haematoloechus sp. JO	14.1	13.3	13.8–15.2	13.6	13.0–13.6	13.6–14.1	14.1–14.7	10.2–12.5	11.1–11.9	12.5–13.0	10.8	–
13. H. complexus	13.2	13.8	15.5–16.3	14.9	11.9–12.5	13.8	14.0	9.9–11.9	13.8–14.1	14.1	15.5	11.91	–
14. H. danbrooksi	9.4–9.7	8.6–9.4	10.9–12.5	11.1–11.4	11.4–13.0	10.3–12.5	13.9–14.4	9.4–10.2	13.8–14.7	12.8–13.3	14.4–15.2	13.3–13.6	13–13.6	–
15. H. longiplexus	16.9–17.1	16.2–16.9	18.0–19.1	18.3	17.2–18.1	17.4–17.7	20.2	14.4–15.2	17.4–18.0	17.7–18.0	19.1	16.6–18.0	16.3	15.2–16.1	–
16. H. macrorchis	16.9	17.2	18.8–19.7	17.7	16.3–16.6	18.0–18.3	19.1	14.6–15.2	17.4	18.0	19.7	17.1–17.7	14.9	14.6–15.2	7.8	–
17. H. exoterorchis	26.3	25.8	29.1–29.6	27.1	27.8–28.2	26.0–26.6	28.2	24.1–24.9	27.7–27.9	27.9	29.3	26.3–26.8	25.5	24.0–24.4	23.3	23.3	–
18. H. micrurus	17.2	17.7	18.6–19.4	17.7	17.4–17.7	18.3–18.6	19.9	17.4–18.0	18.0–18.3	19.9	20.5	17.7–18.2	17.2	16.9–17.1	15.8	14.4	27.1

Note: Minimum and maximum values are shown for groups with multiple sequences and intraspecific variation. O, Oaxaca; V, Veracruz; C, Colima; P, Puebla; and JO, Jalisco-Oaxaca.