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Mitochondrial DNA
The Journal of DNA Mapping, Sequencing, and Analysis
Volume 25, 2014 - Issue 1
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Complete mitochondrial genome of Pseudolaubuca engraulis (Pisces: Cyprinidae, Cultrinae)

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Pages 50-51 | Received 24 Feb 2013, Accepted 28 Feb 2013, Published online: 10 Jul 2013
 

Abstract

The complete mitogenome of Pseudolaubuca engraulis was determined using long-distance polymerase chain reaction and sequenced by primer walking method. It is 16,612 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA (tRNA) genes and 1 A + T-rich control region. The gene arrangement and characterization of P. engraulis are similar to most vertebrates. The overall base composition of the heavy strand is 27.1% A, 27.5% C, 25.9% T and 19.5% G, with a slight A + T bias. The shortest tRNA-Ser2 lacks the dihydrouridine arm and is replaced with a simple loop. The termination-associated sequence and the conserved sequence blocks (1–3) are found in the control region.

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