Genetic differentiation in Indian Major Carp, Cirrhinus mrigala (Hamilton, 1822) from Indian Rivers, as revealed by direct sequencing analysis of mitochondrial Cytochrome b region

Abstract

A 307 bp segment of Cytochrome b gene of mtDNA was sequenced and analyzed for 90 individuals of Cirrhinus mrigala collected across the three rivers, namely Ganges, Narmada and Brahmaputra. Analyses revealed the presence of 14 haplotypes with haplotype diversity (h) ranging from 0.304 to 0.692, and nucleotide diversity (π) 0.002–0.043. The majority of variation was found within the population (96.21%), and the F_ST value (0.035) as well as the value of exact test of population differentiation (0.893) were found to be insignificant (p < 0.05). Analysis of molecular variance (AMOVA) also indicated insignificant differentiation among sub-populations. Generally, low genetic differences were observed even though those populations were from different geographic locations. The present study suggests a single panmictic population of C. mrigala across the three rivers of India.

• Cirrhinus mrigala
• Cytochrome b
• genetic differentiation
• Indian rivers

Acknowledgements

The authors are thankful to Dr S. Ayyappan, Secretary DARE, DG, ICAR, for encouragement. We are also thankful to Mr Deepak Biswas and Mr Asim Kumar Jana for technical assistance rendered.

Declaration of interest

None of the authors claim conflicts of interest. The authors alone are responsible for the content and preparation of this manuscript.