Mitochondrial genomes of the jungle crow Corvus macrorhynchos (Passeriformes: Corvidae) from shed feathers and a phylogenetic analysis of genus Corvus using mitochondrial protein-coding genes

Abstract

The complete mitochondrial genomes of two jungle crows (Corvus macrorhynchos) were sequenced. DNA was extracted from tissue samples obtained from shed feathers collected in the field in Sri Lanka and sequenced using the Illumina MiSeq Personal Sequencer. Jungle crow mitogenomes have a structural organization typical of the genus Corvus and are 16,927 bp and 17,066 bp in length, both comprising 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal subunit genes, and a non-coding control region. In addition, we complement already available house crow (Corvus spelendens) mitogenome resources by sequencing an individual from Singapore. A phylogenetic tree constructed from Corvidae family mitogenome sequences available on GenBank is presented. We confirm the monophyly of the genus Corvus and propose to use complete mitogenome resources for further intra- and interspecies genetic studies.

• Corvus
• large-billed crow
• mitogenome
• Sri Lanka

Declaration of interest

Funding for this study was provided by the Monash University Malaysia Tropical Medicine and Biology Multidisciplinary Platform. Samples in Sri Lanka were collected under the research permit granted by the Department of Wildlife Conservation, Sri Lanka (Permit no. WL/3/2/41/14). Samples from Singapore were provided by the AVA Singapore. The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.