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Research Article

Characterization and cellular interaction of fluorescent-labeled PHEMA nanoparticles

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Pages 78-84 | Received 06 Sep 2012, Accepted 04 Oct 2012, Published online: 10 Jan 2013

Figures & data

Figure 1. a) Size analysis, b) SEM and c) TEM images of AO-loaded nanoparticles.

Figure 1. a) Size analysis, b) SEM and c) TEM images of AO-loaded nanoparticles.

Figure 2. AFM image of AO-loaded PHEMA nanoparticles.

Figure 2. AFM image of AO-loaded PHEMA nanoparticles.

Figure 3. FTIR spectra of a) AO, b) AO-loaded PHEMA nanoparticles c) PHEMA nanoparticles.

Figure 3. FTIR spectra of a) AO, b) AO-loaded PHEMA nanoparticles c) PHEMA nanoparticles.

Figure 4. Fluoresence microscopy images of L929 cells. a) Transmission and b) fluoresence after 24 h incubation at 37°C with AO-loaded PHEMA nanoparticles. Different labeling of cell membranes or compartments can be noticed indicating the release of the AO has occured from polymeric nanoparticles.

Figure 4. Fluoresence microscopy images of L929 cells. a) Transmission and b) fluoresence after 24 h incubation at 37°C with AO-loaded PHEMA nanoparticles. Different labeling of cell membranes or compartments can be noticed indicating the release of the AO has occured from polymeric nanoparticles.

Figure 5. The effect of AO-loaded PHEMA nanoparticle concentration on the cell viability of L929 mouse fibroblast cells.

Figure 5. The effect of AO-loaded PHEMA nanoparticle concentration on the cell viability of L929 mouse fibroblast cells.

Figure 6. TEM images of L929 cells incubated for 24 h with AO-loaded PHEMA nanoparticles showing coagulation of nanoparticles in cell compartments at an accelerating voltage of 120 kV (a, c, d). L929 cells just as control is seen in b).

Figure 6. TEM images of L929 cells incubated for 24 h with AO-loaded PHEMA nanoparticles showing coagulation of nanoparticles in cell compartments at an accelerating voltage of 120 kV (a, c, d). L929 cells just as control is seen in b).

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