Preparation of magnetic gelatin nanoparticles and investigating the possible use as chemotherapeutic agent

Figures & data

Table I. Acetone flow rate effect on both GNP formation yield and particle size.

	Nanoparticle formation efficiency (%)	Mean particle size (diameter, nm)
Acetone flow rate (ml/min)
0.7	79.9 ± 4.0	122.0 ± 5.6
1	83.3 ± 3.8	136.0 ± 4.4
1.3	82.5 ± 4.2	237.0 ± 7.8
1.7	79.5 ± 3.8	234.0 ± 9.3

GNP: Gelatin nanoparticle.

Figure 1. TG analyzes of plain gelatin (A), IONP's (B), GNP's (C) and MGNP's (D).

Figure 2. FTIR curves of plain gelatin (A), IONP's (B), GNP's (C) and MGNP's (D).

Figure 3. Magnetization curves of IONP and MGNP's with different IONP concentration.

Figure 4. (A) SEM image of GNPs’ and (B) TEM image of MGNPs.

Table II. CDDP concentration, contact time and temperature effect on CDDP adsorption onto MGNPs.

	Drug entrapment efficiency (%w/w)
Temperature (°C)*
25	94.6 ± 5.0
30	77.6 ± 5.1
37	78.6 ± 4.8
40	69.2 ± 4.7
Contact time (h)
3	8.7 ± 6.2
6	34.7 ± 5.8
15	40.5 ± 5.0
24 48 CDDP concentration (μg/ml)** 300 350 400 450	62.1 ± 4.9 94.6 ± 5.0 68.4 ± 5.0 71.7 ± 4.9 62.0 ± 4.7 57.5 ± 4.8
500	53.1 ± 5.3

CDDP: Cisplatin, MGNP: Magnetic gelatin nanoparticle.

*For temperature study, CDDP adsorption time was 48 hours based on the earlier study [13].

**For CDDP concentration study, adsorption time was chosen as 24 h owing to the possibility of contamination risk.

Table III. Langmiur, Freundlich and Dubinin-Raduskevich isotherms constants for adsorption of CDDP by MGNPs.

Langmiur constants				Freundlich constants			Dubinin-Raduskevich constants
Q0 (mol/g)	b (L/mol)	R^2	RL	Kf (mol^1-1/n L^1/n g^21)	n	R^2	qm (mol/g)	E (kj/mol)	β	R^2
40.0	122.2	0.8673	0.021	0.002	2.3	0.9886	0.0003	11.0	− 0.0041	0.9802

CDDP: Cisplatin, MGNP: Magnetic gelatin nanoprticle.

Figure 5. Langmiur isotherm (A), Freundlich isotherm (B) and Dubinin-Raduskevich isotherm (C) of CDDP adsorption.

Figure 6. (A) CDDP release from MGNPs’ in different pH (10 mM PBS) and (B) free CDDP release in different pH (10 mM PBS).

Jahanshahı M, Sanatı MH, Hajızadeh S, Babaeı Z. 2008. Gelatin nanoparticle fabrication and optimization of the particle size. Phys Status Solidi A. 205:2898–2902.

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