Development of gemcitabine-adsorbed magnetic gelatin nanoparticles for targeted drug delivery in lung cancer

Figures & data

Figure 1. Sem image of M-GNPs.

Table I. Effect of gemcitabine concentration, contact time, pH, and temperature on gemcitabine adsorption onto M-GNPs. M-GNP: Magnetic gelatin nanoparticle.

	Drug Adsorption Efficiency (%w/w)
Temperature (°C)*
4	8.1 ± 4.5
25	23.1 ± 5.8
30	7.2 ± 6.1
40	2.2 ± 5.4
Contact time (h)
3	54.2 ± 4.9
6	57.8 ± 3.1
9	57.3 ± 4.4
15	61,6 ± 0.4
24	54.1 ± 2.3
Drug concentration (μg/ml)**
50	68.0 ± 6.0
100	39.8 ± 2.2
200	50.2 ± 0.4
200250	52.6 ± 0.4
300	60.6 ± 0.5
350	59.0 ± 2.0
pH
3	66.33 ± 8.7
4	74.09 ± 3.8
5	70.42 ± 0.5
6	55.59 ± 8.5
7	55.43 ± 5.3
8	53.90 ± 11.9

Figure 2. Langmuir isotherm curve of gemcitabine adsorption on nanoparticles.

Table II. Langmuir isotherm constants for adsorption of gemcitabine by M-GNPs. M-GNP: Magnetic gelatin nanoparticle.

	Langmuir constants
	Q0 (mol/g)	b (L/mol)	R^2	RL
25°C	83.8	0.011	0.939	0.768
37°C	142.8	0.0019	0.894	0.894

Table III. Thermodynamic constants for adsorption of gemcitabine by M-GNPs. M-GNP: Magnetic gelatin nanoparticle.

	25°C		37°C
ΔG° (kJ/mol)	−4.74		−7.86
ΔH° (kJ/mol)	–	112.4	–
ΔS° (J/molK)	393.1		387.9

Figure 3. Pseudo-second order kinetic of gemcitabine adsorption on nanoparticles.

Figure 4. Intra-particle kinetic curve of gemcitabine adsorption on nanoparticles.

Table IV. Pseudo-second-order and intra-particle diffusion kinetic constants for adsorption of gemcitabine by M-GNPs. M-GNP: Magnetic gelatin nanoparticle.

	Pseudo-second order			Intra-particle diffusion
	k1	qe	R^2	kp	c	R^2
37°C	0.656	139.9	0.916	11.48	62.5	0.982
25°C	0.661	63.7	0.974	1.48	21.8	0.912

Figure 5. Cumulative drug release of nanoparticles and free drug at pH 6 and pH 7.4.