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Artificial Cells, Nanomedicine, and Biotechnology
An International Journal
Volume 44, 2016 - Issue 1
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ORIGINAL ARTICLE

Preparation and evaluation of multifunctional envelope-type nano device loading siRNA

Hongli YinSchool of Pharmacy, Qingdao University, Qingdao, P. R. China
,
Aizhen MuDepartment of Anesthesiology, Weifang People's Hospital, Weifang, P. R. China
,
Yong SunSchool of Pharmacy, Qingdao University, Qingdao, P. R. ChinaCorrespondence[email protected]
,
Guowen YanSchool of Pharmacy, Qingdao University, Qingdao, P. R. China
,
Haitian WeiSchool of Pharmacy, Qingdao University, Qingdao, P. R. China
,
Chao MaSchool of Pharmacy, Qingdao University, Qingdao, P. R. China
&
Changgang SunDepartment of Oncology, Haici Hospital, Qingdao, P. R. China
 show all
Pages 20-26 | Received 30 Dec 2013, Accepted 26 Mar 2014, Published online: 16 May 2014

Figures & data

Figure 1. Gel retardation assay of siRNA/PLL complex. Lane 1, naked siRNA; Lane 2, PLL; Lane 3, ˜11 mass ratio of PLL to siRNA was 1, 3, 5, 8, 10, 15, 30, 40, and 50 respectively.
Figure 1. Gel retardation assay of siRNA/PLL complex. Lane 1, naked siRNA; Lane 2, PLL; Lane 3, ˜11 mass ratio of PLL to siRNA was 1, 3, 5, 8, 10, 15, 30, 40, and 50 respectively.
Figure 2. Diagram of liner relation between FAM-siRNA concentration and fluorescence intensity.
Figure 2. Diagram of liner relation between FAM-siRNA concentration and fluorescence intensity.

Table I. Factors and levels of orthogonal tests.

Table II. Arrangement and results of orthogonal tests according to L9(34).

Figure 3. Trends of levels in every factor. A, B, C, D means 4 factors and 1, 2, 3 means 3 levels.
Figure 3. Trends of levels in every factor. A, B, C, D means 4 factors and 1, 2, 3 means 3 levels.
Figure 4. Shapes of MEND through TEM.
Figure 4. Shapes of MEND through TEM.
Figure 5. The size distribution and zeta potential distribution of MEND. (A) size distribution of MEND (CHEMS), (B) size distribution of MEND (PLL), (C) zeta potential distribution of MEND (CHEMS), (D) zeta potential distribution of MEND (PLL).
Figure 5. The size distribution and zeta potential distribution of MEND. (A) size distribution of MEND (CHEMS), (B) size distribution of MEND (PLL), (C) zeta potential distribution of MEND (CHEMS), (D) zeta potential distribution of MEND (PLL).

Table III. Particle size and of zeta potential MEND.

Figure 6. SiRNA serum stability experiments. Lane 1, Marker; Lane 2, pure siRNA; Lane 3, siRNA-PLL; Lane 4, siRNA in MEND.
Figure 6. SiRNA serum stability experiments. Lane 1, Marker; Lane 2, pure siRNA; Lane 3, siRNA-PLL; Lane 4, siRNA in MEND.
Figure 7. Results of cell transfection. (A) MEND (PLL) at a concentration of 500 nM FAM-siRNA, (B) MEND (PLL) at a concentration of 250 nM FAM-siRNA, (C) pure FAM-siRNA at a concentration of 500 nM, (D) pure FAM-siRNA at a concentration of 500 nM.
Figure 7. Results of cell transfection. (A) MEND (PLL) at a concentration of 500 nM FAM-siRNA, (B) MEND (PLL) at a concentration of 250 nM FAM-siRNA, (C) pure FAM-siRNA at a concentration of 500 nM, (D) pure FAM-siRNA at a concentration of 500 nM.

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