Necrotic regions are absent in fiber-shaped cell aggregates, approximately 100 μm in diameter

Figures & data

Figure 1. Schematic depiction of calcium alginate hydrogel hollow microfiber formation.

Figure 2. Effects of outer cylinder tip id [910 μm (circles), 610 μm (triangles) and 240 μm (squares)] and flow rates of core and sheath solutions at the tip on od (a) and i/o ratio (b) of the microfibers.

Figure 3. Effect of core volume ratio on microfiber od (closed symbols) and id (open symbols). Two outer cylinders with different tip id (910 μm: circles, 240 μm: triangles) were used.

Figure 4. HepG2 cell-enclosed thick (a, c) and thin (b, d) microfibers at 0 (a, b) and 7 (c, d) day(s) of culture. Arrows show encapsulated cells. Bars represent 500 μm.

Figure 5. Hematoxylin and eosin-stained sections of the cell aggregates formed in thick (a) and thin (b) microfibers. The area surrounded by dotted-line in (a) is the necrotic region. Bars represent 200 μm.