Inhibition of BK_Ca channel currents in vascular smooth muscle cells contributes to HBOC-induced vasoconstriction

Figures & data

Figure 1. The net tension of femoral artery, carotid artery and coronary artery after incubation with HBOC. Values are presented as mean ± SD (n = 5 to 6 per group). *P < 0.05 versus the coronary artery.

Figure 2. (A) Representative image of whole-cell outward current recording by a patch-clamp technique. Scale bar: 30 μm. (B) Whole-cell BK_Ca currents recorded from isolated VSMCs before (baseline) and after inhibition with 100 nM IBTX. (C) Current–voltage relationship of BK_Ca currents with low Ca²⁺ or high Ca²⁺. Values are presented as mean ± SD (n = 6 per group).

Figure 3. Quantification analysis of the changes of BK_Ca currents after exposure to 0.1%, 0.5%, 1%, 2% and 3% HBOC. Values are presented as mean ± SD (n = 6 per group). **P < 0.01 and ***P < 0.001 versus the 0.1%HBOC group.

Figure 4. The linear regression analysis of the relationship between HBOC-induced inhibition of BK_Ca channel currents and net tension of coronary artery.

Table I. Linear regression analysis between BK_Ca channel inhibition and vasoconstriction.

	Femoral artery	Carotid artery	Coronary artery
Equation	y = − 0.002x + 0.002	y = − 0.0027x + 0.0011	y = − 0.004x + 0.0071
Related coefficient	0.9285	0.9186	0.9316