An optimal polymerization process for low mean molecular weight HBOC with lower dimer

Figures & data

Figure 1. Effects of poly-Hb with the reaction time (n = 3).

Table I. Effects of mean molecular weight (kD) of poly-Hb.

Group	A (%)	B(ml/min)	C	1	2	3	Average
1	1	1	1	110.33	114.45	110.34	111.73
2	1	2	2	130.43	138.07	129.76	132.77
3	1	3	3	148.87	162.65	159.65	157.00
4	2	1	3	152.32	149.67	149.74	150.54
5	2	2	1	117.21	117.98	115.54	116.92
6	2	3	2	166.43	156.98	158.87	160.73
7	3	1	2	119.76	120.98	121.43	120.74
8	3	2	3	161.10	165.11	162.65	162.92
9	3	3	1	141.73	138.96	139.65	140.02

Ave1 133.83 127.67 122.89

Ave2 142.73 137.54 138.08

Ave3 141.23 152.58 156.82

Ex D 8.90 24.91 33.93

Table II. Effects of the content of super-weight molecular (%) of poly-Hb.

Group	A (%)	B (ml/min)	C	1	2	3	Average
1	1	1	1	0	0	0	0
2	1	2	2	0	0.42	0.11	0.18
3	1	3	3	1.04	2.21	1.09	1.45
4	2	1	3	0.07	0.29	0.06	0.14
5	2	2	1	0	0	0	0
6	2	3	2	1.53	0.98	1.32	1.28
7	3	1	2	0	0	0	0
8	3	2	3	0.19	0.77	1.21	0.72
9	3	3	1	0	0.06	0	0.02

Ave1 0.05 0.67 0.01

Ave2 0.30 0.11 0.49

Ave3 0.92 0.48 0.77

Ex D 0.87 0.554 0.76

Table III. Content of dimer(%) of poly-Hb.

	A (%)	B (ml/min)	C	1	2	3	Average
1	1	1	1	35.14	34.68	33.69	34.50
2	1	2	2	27.08	26.75	27.81	27.21
3	1	3	3	19.70	16.87	18.93	18.50
4	2	1	2	25.53	25.31	25.68	25.51
5	2	2	1	28.94	27.29	26.50	27.58
6	2	3	3	14.36	18.47	15.65	16.16
7	3	1	3	30.13	27.02	22.63	26.59
8	3	2	1	31.76	30.86	30.80	31.14
9	3	3	2	35.06	32.10	35.03	34.06

Ave1 26.74 28.87 32.05

Ave2 23.08 28.64 23.32

Ave3 30.60 22.91 25.05

Ex D 7.51 5.96 8.73

Table IV. The molecular weight distribution of poly-Hb and final products based on optimal process (n = 3).

Detection index	Poly-Hb	Final products
Average molecular weight (kD)	155.54 ± 5.79	231.57 ± 15.72
Content of dimer (%)	17.23 ± 3.71	8.45 ± 2.19
Content of super-weight molecular (%)	0.17 ± 0.09	1.09 ± 0.74

Figure 2. Secondary structure of final products with circular dichroism (A). Compared with the secondary structure of the stroma-free human placenta Hb (B), the secondary structure of final products is consistent.