Synergistic antiproliferative effects of methotrexate-loaded smart silica nanocomposites in MDA-MB-231 breast cancer cells

Figures & data

Table I. Sequence of primers.

Primers Name	Sequence of Primers	T annealing°C
GAPDH F	5′AAGCTCATTTCCTGGTATGACAACG3′	60
GAPDH R	5′TCTTCCTCTTGTGCTCTTGCTGCTGG3′	60
Bax F	5′ATGGACGGGTCCGGGGAGCA3′	57
Bax R	5′CCCAGTTGAAGTTGCCGTCA3′	57
BaK F	5′CCTTGAGACTCAGTATCGCA3′	57
BaK R	5′CCGGTCAAACACCTAGCTTC3′	57
Bcl-2 F	5′CCTGTGGATGACTGAGTACC3′	57
Bcl-2 R	5′GAGACAGCCAGGAGAAATCA3′	57

Figure 1. Synthesis root of stimuli-responsive and cationic NIPMACIL@MSNs nanocomposites.

Figure 2. Scanning electron micrographs of pH and thermo-responsive silica nanocomposites (NIPMACIL@MSNs).

Figure 3. Schematic root of pH-dependent MTX loading on stimuli-responsive cationic NIPMADM-MSNs.

Figure 4. Cumulative release of MTX from NIPMADM-MSNs at pH 5.5 and 7.4 (at 37°C).

Figure 5. Cell growth inhibition rates by different concentrations of drug-free NIPMACIL@MSN nanocomposites, MTX and MTX@NIPMACIL@MSN nanocomposites.

Figure 6. qRT-PCR analysis of Bcl-2, synergistic effect of MTX and MTX@NIPMACIL@MSNs, in different concentrations on Bcl-2 expression. Bcl-2 mRNA level was more abundantly down-regulated in cultured cells, in 800 μg/Ml of MTX@NIPMACIL@MSNs (4 mg/ml Silica nano composite and 0.8 mg/ml Methotrexate) concentration shows more Bcl-2 mRNA level down-regulation.

Figure 7. qRT-PCR analysis of Bax, synergistic effect of MTX and MTX@NIPMACIL@MSNs, in different concentrations on Bax expression. Bax mRNA level was more abundantly up regulated in cultured cells, in 800 μg/Ml of MTX@NIPMACIL@MSNs (4 mg/ml Silica nano composite and 0.8 mg/ml Methotrexate) concentration shows more Bax mRNA level up-regulation.