Molecularly imprinted cryogels for chondroitin sulfate recognition

Figures & data

Figure 1. SEM images of A) CsMIP, B) NIP cryogel.

Figure 2. FTIR spectrum of Cs, VIM and VIM-Cu(II)-Cs precomplex.

Figure 3. FTIR spectrum of CsMIP and NIP columns.

Figure 4. Effect of equilibrium concentration of Cs on the Cs adsorption amount, and the Langmuir and Freundlich adsorption isotherms. V: 5 mL, Mdry: 0.263 g.

Table I. Langmuir and Freundlich adsorption isotherm constants and correlation coefficients for Cs.

Experimental Qexp (mg/g)	Langmuir constants			Freundlich constants
	Qmax (mg/g)	b (g/mL)	R^2	Qf (mg/g)	n	R^2
3.40	3.05	1.74	0.97	4.68	0.62	0.91

Figure 5. Reusability of the CsMIP cryogel column. Cycle number 10, V: 5 mL, Cs: 0.2 mg/mL, Mdry: 0.263 g.

Figure 6. Amount of compounds adsorbed onto the CsMIP and NIP columns. Ceq: 2 mg/mL, V: 5 mL, t: 120 min, Mdry: 0.63 g.

Table II. Selectivity constant values (k, K_d, k’) of Cs.

Compounds	Nip		CsMip
	K d	K	K d	K	k′
Cs	1.3		3.02
Hp	1.45	0.89	1.67	1.81	2.03

Figure 7. Fast protein liquid chromatography (FPLC) system separation of Cs from aqueous Cs-Hp solution.

Table III. Chromatographic separation data of the FPLC study.

	tR (min)	N	Cf	A	R s
Cs	9.25	6744	34.57	3.81	14.72
Hp	2.63	447	9.07	–	–