Separation and determination of reduced vitamin C in polymerized hemoglobin-based oxygen carriers of the human placenta

Figures & data

Figure 1. A₆₅₂ of the supernatants with different HCl concentrations, after centrifugation.

Figure 2. The absorbance curve of the coloring solution between 500 and 900 nm.

Figure 3. Effects of amounts of HCl added, on the value of A_710.

Figure 4. Effects of time on the coloring reaction.

Figure 5. The standard curve of Vc testing.

Table I. Results of sensitivity experiments.

Vc quantity(μg)	0	2	4	6	8	10	12
A710-1	0.005	0.007	0.008	0.008	0.008	0.013	0.017
A710-2	0.007	0.005	0.006	0.007	0.009	0.013	0.014
A710-3	0.004	0.009	0.007	0.007	0.007	0.011	0.015
A710-av	0.005	0.007	0.007	0.007	0.008	0.012	0.015

Table II. Results of repeatability experiments.

	A710-1	A710-2	A710-3	A710-4	A710-5	A710-6	RSD值
Sample A	0.212	0.201	0.214	0.200	0.205	0.200	3.24%
Sample B	0.112	0.118	0.107	0.116	0.114	0.110	3.76%
Sample C	0.025	0.024	0.025	0.027	0.026	0.025	4.54%

Table III. Results of precision experiments.

	A710-1	A710-2	A710-3	A710-4	A710-5	A710-6	RSD值
Sample A	0.218	0.223	0.217	0.207	0.209	0.225	3.26%
Sample B	0.160	0.159	0.155	0.159	0.164	0.166	2.30%
Sample C	0.035	0.034	0.034	0.034	0.036	0.036	2.81%

Table IV. Results of recovery experiments.

Samples	1	2	3	References
Spiked amount of Vc addition(mg/ml)	1.00	0.50	0.20	0.12
Detected amount of total Vc addition(mg/ml)	1.14	0.61	0.31
Detected amount of standard Vc addition (mg/ml)	1.02	0.49	0.19
Recovery	101.81%	97.93%	97.02%

Average of 3 experiments.